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Literature DB >> 24213157

Transformation and regeneration of Brassica rapa using Agrobacterium tumefaciens.

Abstract

Transformation and regeneration procedures for obtaining transgenic Brassica rapa ssp. oleifera plants are described. Regeneration frequencies were increasedby using silver nitrate and by adjusting the duration of exposure to 2,4-D. For transformation, Agrobacterium tumefaciens strain EHA101 containing a binary plasmid with the neomycin phosphotransferase gene (NPT II) and the b-glucuronidase gene (GUS) was cocultivated with hypocotyl explants from the oilseed B. rapa cvs. Tobin and Emma. Transformed plants were obtained within three months of cocultivation. Transformation frequencies for the cultivars Tobin and Emma were 1-9%. Evidence for transformation was shown by NPT II dot blot assay, the GUS fluorometric assay, Southern analysis, and segregation of the kanamycin-resistance trait in the progeny. The transformation and regeneration procedure described here has been used routinely to transform two cultivars of B. rapa and 18 cultivars of B. napus.

Entities: Chemical Species

Year: 1992 PMID： 24213157 DOI： 10.1007/BF00236265

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

12 in total

1. Novel and useful properties of a chimeric plant promoter combining CaMV 35S and MAS elements.

Authors: L Comai; P Moran; D Maslyar
Journal: Plant Mol Biol Date: 1990-09 Impact factor: 4.076

2. Accumulation of a Brazil nut albumin in seeds of transgenic canola results in enhanced levels of seed protein methionine.

Authors: S B Altenbach; C C Kuo; L C Staraci; K W Pearson; C Wainwright; A Georgescu; J Townsend
Journal: Plant Mol Biol Date: 1992-01 Impact factor: 4.076

3. Efficient shoot regeneration of Brassica campestris using cotyledon explants cultured in vitro.

Authors: J E Hachey; K K Sharma; M M Moloney
Journal: Plant Cell Rep Date: 1991-02 Impact factor: 4.570

4. Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene.

Authors: D S Knutzon; G A Thompson; S E Radke; W B Johnson; V C Knauf; J C Kridl
Journal: Proc Natl Acad Sci U S A Date: 1992-04-01 Impact factor: 11.205

5. The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA.

Authors: E E Hood; G L Helmer; R T Fraley; M D Chilton
Journal: J Bacteriol Date: 1986-12 Impact factor: 3.490

6. Gene transfer in plants of Brassica juncea using Agrobacterium tumefaciens-mediated transformation.

Authors: D G Barfield; E C Pua
Journal: Plant Cell Rep Date: 1991-09 Impact factor: 4.570

7. Effect of AgNO3 and aminoethoxyvinylglycine on in vitro shoot and root organogenesis from seedling explants of recalcitrant Brassica genotypes.

Authors: G L Chi; D G Barfield; G E Sim; E C Pua
Journal: Plant Cell Rep Date: 1990-08 Impact factor: 4.570

8. Stimulation of shoot regeneration in Triticum aestivum and Nicotiana plumbaginifolia Viv. tissue cultures using the ethylene inhibitor AgNO3.

Authors: L Purnhauser; P Medgyesy; M Czakó; P J Dix; L Márton
Journal: Plant Cell Rep Date: 1987-02 Impact factor: 4.570

9. Transformation and regeneration of Brassica oleracea mediated by an oncogenic Agrobacterium tumefaciens.

Authors: V Srivastava; A S Reddy; S Guha-Mukherjee
Journal: Plant Cell Rep Date: 1988-12 Impact factor: 4.570

10. Improved binary vectors for Agrobacterium-mediated plant transformation.

Authors: K E McBride; K R Summerfelt
Journal: Plant Mol Biol Date: 1990-02 Impact factor: 4.076

10 in total

1. Enhanced production of single copy backbone-free transgenic plants in multiple crop species using binary vectors with a pRi replication origin in Agrobacterium tumefaciens.

Authors: Xudong Ye; Edward J Williams; Junjiang Shen; Susan Johnson; Brenda Lowe; Sharon Radke; Steve Strickland; James A Esser; Michael W Petersen; Larry A Gilbertson
Journal: Transgenic Res Date: 2010-11-02 Impact factor: 2.788

2. The promoter of aBrassica napus polygalacturonase gene directs pollen expression ofβ-glucuronidase in transgenicBrassica plants.

Authors: H P Hong; J L Gerster; R S S Datla; D Albani; G Scoles; W Keller; L S Robert
Journal: Plant Cell Rep Date: 1997-03 Impact factor: 4.570

3. High-level production of gamma-linolenic acid in Brassica juncea using a delta6 desaturase from Pythium irregulare.

Authors: Haiping Hong; Nagamani Datla; Darwin W Reed; Patrick S Covello; Samuel L MacKenzie; Xiao Qiu
Journal: Plant Physiol Date: 2002-05 Impact factor: 8.340

4. Variation amongst Brassica juncea cultivars for regeneration from hypocotyl explants and optimization of conditions for Agrobacterium-mediated genetic transformation.

Authors: D Pental; A K Pradhan; Y S Sodhi; A Mukhopadhyay
Journal: Plant Cell Rep Date: 1993-05 Impact factor: 4.570

5. Ethylene precursors and antagonists increase embryogenesis of Hordeum vulgare L. anther culture.

Authors: J M Evans; N P Batty
Journal: Plant Cell Rep Date: 1994-09 Impact factor: 4.570

6. Towards the production of high levels of eicosapentaenoic acid in transgenic plants: the effects of different host species, genes and promoters.

Authors: Bifang Cheng; Guohai Wu; Patricia Vrinten; Kevin Falk; Joerg Bauer; Xiao Qiu
Journal: Transgenic Res Date: 2009-07-07 Impact factor: 2.788