Literature DB >> 30727644

The promoter of aBrassica napus polygalacturonase gene directs pollen expression ofβ-glucuronidase in transgenicBrassica plants.

H P Hong1, J L Gerster2, R S S Datla1, D Albani1, G Scoles3, W Keller1, L S Robert2.   

Abstract

A 647-bp 5'-flanking fragment obtained from genomic clone Sta 44G(2) belonging to a family of polygalacturonase genes expressed inBrassica napus pollen was fused to theβ-glucuronidase (GUS) marker gene. This fusion construct was introduced intoB. napus plants viaAgrobacterium tumefaciens transformation. Analysis of the transgenicB. napus plants revealed that this promoter fragment is sufficient to direct GUS expression specifically in the anther and that GUS activity increases in pollen during maturation.

Entities:  

Keywords:  Brassica; GUS; Pollen; Polygalacturonase; Promoter; Transformation

Year:  1997        PMID： 30727644     DOI： 10.1007/BF01146776

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  27 in total

1.  Modified binary plant transformation vectors with the wild-type gene encoding NPTII.

Authors:  R S Datla; J K Hammerlindl; B Panchuk; L E Pelcher; W Keller
Journal:  Gene       Date:  1992-12-15       Impact factor: 3.688

2.  Preferential expression of an alpha-tubulin gene of Arabidopsis in pollen.

Authors:  J L Carpenter; S E Ploense; D P Snustad; C D Silflow
Journal:  Plant Cell       Date:  1992-05       Impact factor: 11.277

3.  Characterization of a pollen-specific gene family from Brassica napus which is activated during early microspore development.

Authors:  D Albani; L S Robert; P A Donaldson; I Altosaar; P G Arnison; S F Fabijanski
Journal:  Plant Mol Biol       Date:  1990-10       Impact factor: 4.076

4.  A new allele of the duplicated 27kD zein locus of maize generated by homologous recombination.

Authors:  O P Das; E Poliak; K Ward; J Messing
Journal:  Nucleic Acids Res       Date:  1991-06-25       Impact factor: 16.971

5.  A novel wheat alpha-amylase gene (alpha-Amy3).

Authors:  D C Baulcombe; A K Huttly; R A Martienssen; R F Barker; M G Jarvis
Journal:  Mol Gen Genet       Date:  1987-08

6.  Molecular analysis of tap2, an anther-specific gene from Antirrhinum majus.

Authors:  W K Nacken; P Huijser; H Saedler; H Sommer
Journal:  FEBS Lett       Date:  1991-03-11       Impact factor: 4.124

7.  Nucleotide Sequence of a Soybean Vegetative Storage Protein vspA Gene.

Authors:  Y Rhee; P E Staswick
Journal:  Plant Physiol       Date:  1992-02       Impact factor: 8.340

8.  Promoters of auxin-induced genes from tobacco can lead to auxin-inducible and root tip-specific expression.

Authors:  E J van der Zaal; F N Droog; C J Boot; L A Hensgens; J H Hoge; R A Schilperoort; K R Libbenga
Journal:  Plant Mol Biol       Date:  1991-06       Impact factor: 4.076

9.  Structure and function of the promoter of the carrot V-type H(+)-ATPase catalytic subunit gene.

Authors:  I Struve; T Rausch; P Bernasconi; L Taiz
Journal:  J Biol Chem       Date:  1990-05-15       Impact factor: 5.157

10.  Isolation and analysis of a genomic clone encoding a pokeweed antiviral protein.

Authors:  J Kataoka; N Habuka; C Masuta; M Miyano; A Koiwai
Journal:  Plant Mol Biol       Date:  1992-12       Impact factor: 4.076
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