Literature DB >> 2420487

Two-site time-resolved immunofluorometric assay of human insulin.

E Toivonen, I Hemmilä, J Marniemi, P N Jørgensen, J Zeuthen, T Lövgren.   

Abstract

We describe a two-site "sandwich"-type time-resolved immunofluorometric assay for human insulin, based on use of two monoclonal antibodies with different specificities. The first antibody is immobilized on the surface of microtiter plate strip wells, the other is labeled with Eu3+. Serum samples can be assayed with one incubation step; two incubation steps are required when plasma samples are assayed. After the immunoreactions are complete, the bound fraction of Eu3+-label is quantified by dissociating it in a fluorescence-enhancement solution and measuring its fluorescence with a fluorometer with time-resolution. The sensitivity of the assay is 0.24 micro-int. units/mL. The standard curve is linear from 0.24 to 2400 micro-int. units/mL.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 2420487

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


  9 in total

1.  Immunocytochemical localization and immunochemical characterization of an insulin-related peptide in the insect Leucophaea maderae.

Authors:  G N Hansen; B L Hansen; P N Jørgensen; B Scharrer
Journal:  Cell Tissue Res       Date:  1990-02       Impact factor: 5.249

2.  A sensitive and reliable method for assaying true human insulin without interaction with human proinsulin-like molecules.

Authors:  L D Monti; E P Sandoli; V C Phan; P M Piatti; S Costa; A Secchi; G Pozza
Journal:  Acta Diabetol       Date:  1995-03       Impact factor: 4.280

3.  Immunocytochemical localization and immunochemical characterization of an insulin-related peptide in the pancreas of the urodele amphibian, Ambystoma mexicanum.

Authors:  G N Hansen; B L Hansen; P N Jørgensen; C K Vogel
Journal:  Cell Tissue Res       Date:  1989-06       Impact factor: 5.249

4.  Sensitive and specific two-site immunoradiometric assays for human insulin, proinsulin, 65-66 split and 32-33 split proinsulins.

Authors:  W J Sobey; S F Beer; C A Carrington; P M Clark; B H Frank; I P Gray; S D Luzio; D R Owens; A E Schneider; K Siddle
Journal:  Biochem J       Date:  1989-06-01       Impact factor: 3.857

5.  Serum saturated fatty acids containing triacylglycerols are better markers of insulin resistance than total serum triacylglycerol concentrations.

Authors:  A Kotronen; V R Velagapudi; L Yetukuri; J Westerbacka; R Bergholm; K Ekroos; J Makkonen; M-R Taskinen; M Oresic; H Yki-Järvinen
Journal:  Diabetologia       Date:  2009-02-13       Impact factor: 10.122

6.  Insulin binding to the analytical antibody sandwich pair OXI-005 and HUI-018: Epitope mapping and binding properties.

Authors:  Eva Johansson; Xiaoai Wu; Bingke Yu; Zhiru Yang; Zheng Cao; Charlotte Wiberg; Claus B Jeppesen; Fritz Poulsen
Journal:  Protein Sci       Date:  2020-12-15       Impact factor: 6.993

7.  Anguli Parimana in Ayurveda and its association with adiposity and diabetes.

Authors:  Jyoti A Shirodkar; Mehmood G Sayyad; Vilas M Nanal; Chittaranjan S Yajnik
Journal:  J Ayurveda Integr Med       Date:  2014-07

8.  Relationships of maternal and paternal birthweights to features of the metabolic syndrome in adult offspring: an inter-generational study in South India.

Authors:  S R Veena; S Geetha; S D Leary; J Saperia; D J Fisher; K Kumaran; P Coakley; C E Stein; C H D Fall
Journal:  Diabetologia       Date:  2006-12-02       Impact factor: 10.122

9.  Vitamin B12 and folate concentrations during pregnancy and insulin resistance in the offspring: the Pune Maternal Nutrition Study.

Authors:  C S Yajnik; S S Deshpande; A A Jackson; H Refsum; S Rao; D J Fisher; D S Bhat; S S Naik; K J Coyaji; C V Joglekar; N Joshi; H G Lubree; V U Deshpande; S S Rege; C H D Fall
Journal:  Diabetologia       Date:  2007-09-13       Impact factor: 10.122
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.