Literature DB >> 24197428

Dissociation of yeast ribosomes; The effects of hydrostatic pressure and KCl.

W J Amesz1, E M Bevers, H P Bloemers.   

Abstract

The dissociation of free ribosomes at elevated concentrations of KCl is dependent on hydrostatic pressure. The pressure necessary for the dissociation is determined for KCl concentrations ranging from 0.1-0.4 M. It varies between 425 kg cm(-2) at 0.1 M and 10 kg cm(-2) at 0.4 M. The partial dissociation of complex ribosomes in KCl is dependent on hydrostatic pressure in the same way as the complete dissociation of free ribosomes. Therefore, it is concluded that mRNA and peptidyl-tRNA do not contribute to the stability of the ribosome under these conditions.

Entities:  

Year:  1973        PMID： 24197428     DOI： 10.1007/BF00357403

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  15 in total

1.  Association products of native and derived ribosomal subunits of E. coli and their stability during centrifugation.

Authors:  O P. van Diggelen; H Oostrom; L Bosch
Journal:  FEBS Lett       Date:  1971-12-01       Impact factor: 4.124

2.  Polyribosomes in rat tissues. IV. On the abnormal dimer peak in hepatomas.

Authors:  T E Webb; V R Potter
Journal:  Cancer Res       Date:  1966-05       Impact factor: 12.701

3.  Isolation of active ribosomal subunits from yeast.

Authors:  B A van der Zeijst; A J Kool; H P Bloemers
Journal:  Eur J Biochem       Date:  1972-10-17

4.  Equivolumetric glycerol and sucrose gradients for the B-XIV and B-XV zonal rotors.

Authors:  B A van der Zeijst; H Bult
Journal:  Eur J Biochem       Date:  1972-08-04

5.  Dissociation of ribosomes induced by centrifugation: evidence for doubting conformational changes in ribosomes.

Authors:  A A Infante; M Krauss
Journal:  Biochim Biophys Acta       Date:  1971-08-12

6.  Analytical studies on nuclear ribonucleic acid using polyacrylamide gel electrophoresis.

Authors:  C W Dingman; A C Peacock
Journal:  Biochemistry       Date:  1968-02       Impact factor: 3.162

7.  Resistance of active yeast ribosomes to dissociation by KCl.

Authors:  T E Martin; L H Hartwell
Journal:  J Biol Chem       Date:  1970-03-25       Impact factor: 5.157

8.  Pressure-induced dissociation of sedimenting ribosomes: effect on sedimentation patterns.

Authors:  A A Infante; R Baierlein
Journal:  Proc Natl Acad Sci U S A       Date:  1971-08       Impact factor: 11.205

9.  Characterization of macromolecules by constant velocity sedimentation.

Authors:  H Noll
Journal:  Nature       Date:  1967-07-22       Impact factor: 49.962

10.  On the significance of ribosome dimers in extracts of animal cells.

Authors:  R W Reader; C P Stanners
Journal:  J Mol Biol       Date:  1967-09-14       Impact factor: 5.469
View more
  1 in total

1.  Dissociability of free and peptidyl-tRNA bound ribosomes.

Authors:  A P Surguchov; E S Fominykch; L V Lyzlova
Journal:  Mol Biol Rep       Date:  1978-06-16       Impact factor: 2.316
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.