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Literature DB >> 355860

Dissociability of free and peptidyl-tRNA bound ribosomes.

A P Surguchov, E S Fominykch, L V Lyzlova.

Abstract

The influence of peptidyl-tRNA on the dissociation of yeast 80 S ribosomes into subunits was studied. For this purpose temperature-sensitive (ts) suppressor strain of yeast Saccharomyces cervisiae carrying a defect in peptide chain termination was used. It was found that peptidyl-tRNA did not influence the dissociation of ribosomes either at high salt concentration or in the presence of dissociation factor (DF) from yeast. After dissociation of yeast ribosomes in 0.5 M KCl, peptidyl-tRNA remains bound to the 60 S subunit. Some characteristics of the termination process and release of nascent polypeptides from yeast ribosomes are discussed.

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Year: 1978 PMID： 355860 DOI： 10.1007/bf00775964

Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316

11 in total

1. Requirement for ribosome-releasing factor for the release of ribosomes at the termination codon.

Authors: K Ogawa; A Kaji
Journal: Eur J Biochem Date: 1975-10-15

2. Yeast ribosomal proteins are synthesized on small polysomes.

Authors: W H Mager; R J Planta
Journal: Eur J Biochem Date: 1976-02-02

3. Recessive nonsense-suppression in yeast: further characterization of a defect in translation.

Authors: V N Smirnov; A P Surguchov; E S Fominykch; L V Lizlova; T V Saprygina; S G Inge-Vechtomov
Journal: FEBS Lett Date: 1976-07-01 Impact factor: 4.124

4. Peptidyl-puromycin synthesis on polyribosomes from Escherichia coli.

Authors: S Pestka
Journal: Methods Enzymol Date: 1974 Impact factor: 1.600

5. Preparation and assay of ribosome dissociation factors from Escherichia coli and rabbit reticulocytes and the assay for free ribosomes.

Authors: M Gottlieb; N H Lubsen; B D Davis
Journal: Methods Enzymol Date: 1974 Impact factor: 1.600

1. Recessive nonsense-suppression in yeast Saccharomyces cerevisiae: the study of 80 S ribosomes accumulated in suppressor strain under non-permissive conditions.

Authors: A P Surguchov; E S Fominykch; Y V Berestetskaya; V N Smirnov; S G Inge-Vechtomov
Journal: Mol Gen Genet Date: 1980

1 in total

Dissociability of free and peptidyl-tRNA bound ribosomes.

1. Requirement for ribosome-releasing factor for the release of ribosomes at the termination codon.

2. Yeast ribosomal proteins are synthesized on small polysomes.

3. Recessive nonsense-suppression in yeast: further characterization of a defect in translation.

4. Peptidyl-puromycin synthesis on polyribosomes from Escherichia coli.

5. Preparation and assay of ribosome dissociation factors from Escherichia coli and rabbit reticulocytes and the assay for free ribosomes.

6. Recessive super-suppression in yeast.

7. Effect of polypeptide chain length on dissociation of ribosomal complexes.

8. Isolation of active ribosomal subunits from yeast.

9. Translocation in Bacillus subtilis: characterization of elongation factor G by peptidyl-[3H]puromycin synthesis.

10. The ribosome dissociation factor and the ribosome-polysome cycle.

1. Recessive nonsense-suppression in yeast Saccharomyces cerevisiae: the study of 80 S ribosomes accumulated in suppressor strain under non-permissive conditions.