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Literature DB >> 24197301

Segregating random amplified polymorphic DNAs (RAPDs) in Betula alleghaniensis.

A Roy¹, N Frascaria, J Mackay, J Bousquet.

Abstract

Molecular markers are currently being developed for Betula alleghaniensis Britton using random amplified polymorphic DNA (RAPD). Arbitrarily designed 11-mer primers were tested on three intraspecific controlled crosses for which more than 15 full-sibs were available. Using two of these primers, we were able to genetically characterize a total of nine polymorphic RAPD markers. Segregation of these markers was consistent with a biparental diploid mode of inheritance, and all appeared dominant. RAPDs were valuable in detecting contaminants and, therefore, in assessing the validity of controlled crosses. Limitations of the technique are discussed in relation to the determination of parental genotypes and construction of linkage maps for hardwood species.

Entities: Species

Year: 1992 PMID： 24197301 DOI： 10.1007/BF00222856

Source DB: PubMed Journal: Theor Appl Genet ISSN： 0040-5752 Impact factor: 5.699

15 in total

1. DNA amplification fingerprinting using very short arbitrary oligonucleotide primers.

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2. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.

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Journal: Proc Natl Acad Sci U S A Date: 1991-11-01 Impact factor: 11.205

3. Rapid identification of genetic variation and pathotype of Leptosphaeria maculans by random amplified polymorphic DNA assay.

Authors: P H Goodwin; S L Annis
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4. Maximizing sensitivity and specificity of PCR by pre-amplification heating.

Authors: R T D'Aquila; L J Bechtel; J A Videler; J J Eron; P Gorczyca; J C Kaplan
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5. Parentage determination in maize hybrids using the arbitrarily primed polymerase chain reaction (AP-PCR).

Authors: J Welsh; R J Honeycutt; M McClelland; B W Sobral
Journal: Theor Appl Genet Date: 1991-07 Impact factor: 5.699

6. Isolation of molecular markers for tomato (L. esculentum) using random amplified polymorphic DNA (RAPD).

Authors: R M Klein-Lankhorst; A Vermunt; R Weide; T Liharska; P Zabel
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7. Segregation of random amplified DNA markers in F1 progeny of conifers.

Authors: J E Carlson; L K Tulsieram; J C Glaubitz; V W Luk; C Kauffeldt; R Rutledge
Journal: Theor Appl Genet Date: 1991-12 Impact factor: 5.699

8. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

Authors: J G Williams; A R Kubelik; K J Livak; J A Rafalski; S V Tingey
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9. Improved microfluorometric DNA determination in biological material using 33258 Hoechst.

Authors: C F Cesarone; C Bolognesi; L Santi
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10. Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping.

Authors: J Welsh; C Petersen; M McClelland
Journal: Nucleic Acids Res Date: 1991-01-25 Impact factor: 16.971

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2. DNA fragments of organellar origin in random amplified polymorphic DNA (RAPD) patterns of sugar beet (Beta vulgaris L.).

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3. Phylogeographic inferences concerning evolution of Brazilian Passiflora actinia and P. elegans (Passifloraceae) based on ITS (nrDNA) variation.

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4. DNA metabarcoding diet analysis for species with parapatric vs sympatric distribution: a case study on subterranean rodents.

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Journal: Heredity (Edinb) Date: 2015-02-04 Impact factor: 3.821

5. RAPD fingerprinting of blackcurrant (Ribes nigrum L.) cultivars.

Authors: P G Lanham; R M Brennan; C Hackett; R J McNicol
Journal: Theor Appl Genet Date: 1995-02 Impact factor: 5.699

6. RAPD linkage mapping in a longleaf pine x slash pine F1 family.

Authors: T L Kubisiak; C D Nelson; W L Nance; M Stine
Journal: Theor Appl Genet Date: 1995-06 Impact factor: 5.699

7. Pollen dispersal and breeding structure in a hawkmoth-pollinated Pampa grasslands species Petunia axillaris (Solanaceae).

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Journal: Ann Bot Date: 2015-03-25 Impact factor: 4.357

8. MAAP: a versatile and universal tool for genome analysis.

Authors: G Caetano-Anollés
Journal: Plant Mol Biol Date: 1994-09 Impact factor: 4.076

9. RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P.

Authors: N Isabel; L Tremblay; M Michaud; F M Tremblay; J Bousquet
Journal: Theor Appl Genet Date: 1993-03 Impact factor: 5.699

10. Detection of genetic diversity and selective gene introgression in coffee using RAPD markers.

Authors: C Orozco-Castillo; K J Chalmers; R Waugh; W Powell
Journal: Theor Appl Genet Date: 1994-03 Impact factor: 5.699