Literature DB >> 24196288

Transformation of maize (Zea mays L.) protoplasts and regeneration of haploid transgenic plants.

K Sukhapinda1, M E Kozuch, B Rubin-Wilson, W M Ainley, D J Merlo.   

Abstract

Transgenic haploid maize (Zea mays L.) plants were obtained from protoplasts isolated from microspore-derived cell suspension cultures. Protoplasts were electroporated in the presence of plasmid DNA containing the gus A and npt II genes encoding ß-glucuronidase (GUS) and neomycin phosphotransferase II (NPT II), respectively. Transformed calli were selected and continuously maintained on kanamycin containing medium. Stable transformation was confirmed by enzyme assays and DNA. analysis. Stably transformed tissue was transferred to regeneration medium and several plants were obtained. Most plants showed NPT II activity, and some also showed GUS activity. Chromosome examinations performed on representative plants showed that they were haploid. As expected, these plants were infertile.

Entities:  

Year:  1993        PMID： 24196288     DOI： 10.1007/BF00235291

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  21 in total

1.  Introns increase gene expression in cultured maize cells.

Authors:  J Callis; M Fromm; V Walbot
Journal:  Genes Dev       Date:  1987-12       Impact factor: 11.361

2.  Nucleotide sequence of the T-DNA region from theA grobacterium tumefaciens octopine Ti plasmid pTi15955.

Authors:  R F Barker; K B Idler; D V Thompson; J D Kemp
Journal:  Plant Mol Biol       Date:  1983-11       Impact factor: 4.076

3.  Fractionation of plant protoplast types by iso-osmotic density gradient centrifugation.

Authors:  C T Harms; I Potrykus
Journal:  Theor Appl Genet       Date:  1978-09       Impact factor: 5.699

4.  Stable transformation of maize after gene transfer by electroporation.

Authors:  M E Fromm; L P Taylor; V Walbot
Journal:  Nature       Date:  1986 Feb 27-Mar 5       Impact factor: 49.962

5.  Genetically transformed maize plants from protoplasts.

Authors:  C A Rhodes; D A Pierce; I J Mettler; D Mascarenhas; J J Detmer
Journal:  Science       Date:  1988-04-08       Impact factor: 47.728

6.  Transformation of Zea mays L. Using Agrobacterium tumefaciens and the Shoot Apex.

Authors:  J Gould; M Devey; O Hasegawa; E C Ulian; G Peterson; R H Smith
Journal:  Plant Physiol       Date:  1991-02       Impact factor: 8.340

7.  Effect of nurse cultures on the production of macro-calli and fertile plants from maize embryogenic suspension culture protoplasts.

Authors:  W L Petersen; S Sulc; C L Armstrong
Journal:  Plant Cell Rep       Date:  1992-01       Impact factor: 4.570

8.  Efficient production of doubled haploid plants through colchicine treatment of anther-derived maize callus.

Authors:  Y Wan; J F Petolino; J M Widholm
Journal:  Theor Appl Genet       Date:  1989-06       Impact factor: 5.699

9.  Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.

Authors:  W. J. Gordon-Kamm; T. M. Spencer; M. L. Mangano; T. R. Adams; R. J. Daines; W. G. Start; J. V. O'Brien; S. A. Chambers; W. R. Adams; N. G. Willetts; T. B. Rice; C. J. Mackey; R. W. Krueger; A. P. Kausch; P. G. Lemaux
Journal:  Plant Cell       Date:  1990-07       Impact factor: 11.277

10.  Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants.

Authors:  D A Walters; C S Vetsch; D E Potts; R C Lundquist
Journal:  Plant Mol Biol       Date:  1992-01       Impact factor: 4.076
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  3 in total

1.  Transformation of haploid, microspore-derived cell suspension protoplasts of rice (Oryza sativa L.).

Authors:  H Chaïr; T Legavre; E Guiderdoni
Journal:  Plant Cell Rep       Date:  1996-06       Impact factor: 4.570

Review 2.  Molecular improvement of cereals.

Authors:  I K Vasil
Journal:  Plant Mol Biol       Date:  1994-09       Impact factor: 4.076

3.  Stable transformation via electroporation into maize Type II callus and regeneration of fertile transgenic plants.

Authors:  S M Pescitelli; K Sukhapinda
Journal:  Plant Cell Rep       Date:  1995-08       Impact factor: 4.570
  3 in total

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