Literature DB >> 24186758

Agrobacterium-mediated transformation of almond leaf pieces.

T Archilletti1, P Lauri, C Damiano.   

Abstract

A protocol for Agrobacterium-mediated transformation of leaf pieces from two almond cultivars has been developed. This protocol gave a transformation percentage of 48.6 for the cultivar MN51 and 27.9 for the cultivar 'Supernova'. The Agrobacterium tumefaciens strain LBA 4404 carrying a binary vector plasmid pBinGUSint has been used. A critical step for optimal transformation was the pre-culture period of leaf pieces prior selection on medium containing kanamycin. Both cultivars showed an almost doubled transformation percentage when the selection was started at day 6 compared to day 0. The presence of neomycin phosphotransferase II and ß-glucuronidase genes carried by pBinGUSint was confirmed in transformed expiants both by Southern blotting analysis and enzyme activity assays.

Entities:  

Year:  1995        PMID： 24186758     DOI： 10.1007/BF00232026

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  9 in total

1.  Genetically engineering plants for crop improvement.

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Authors:  D J James; A J Passey; D J Barbara; M Bevan
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3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
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4.  Nutrient requirements of suspension cultures of soybean root cells.

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5.  Kanamycin-resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8 and pEMBL9.

Authors:  B G Spratt; P J Hedge; S te Heesen; A Edelman; J K Broome-Smith
Journal:  Gene       Date:  1986       Impact factor: 3.688

6.  Regeneration of transgenic plants of Prunus armeniaca containing the coat protein gene of Plum Pox Virus.

Authors:  M L da Câmara Machado; A da Câmara Machado; V Hanzer; H Weiss; F Regner; H Steinkellner; D Mattanovich; R Plail; E Knapp; B Kalthoff; H Katinger
Journal:  Plant Cell Rep       Date:  1992-02       Impact factor: 4.570

7.  Binary Agrobacterium vectors for plant transformation.

Authors:  M Bevan
Journal:  Nucleic Acids Res       Date:  1984-11-26       Impact factor: 16.971

8.  Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation.

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Journal:  Mol Gen Genet       Date:  1990-01

9.  Parameters affecting the frequency of kanamycin resistant alfalfa obtained by Agrobacterium tumefaciens mediated transformation.

Authors:  M Chabaud; J E Passiatore; F Cannon; V Buchanan-Wollaston
Journal:  Plant Cell Rep       Date:  1988-12       Impact factor: 4.570
  9 in total
  7 in total

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Journal:  Plant Cell Rep       Date:  2006-03-14       Impact factor: 4.570

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  7 in total

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