Literature DB >> 24240404

Parameters affecting the frequency of kanamycin resistant alfalfa obtained by Agrobacterium tumefaciens mediated transformation.

M Chabaud1, J E Passiatore, F Cannon, V Buchanan-Wollaston.   

Abstract

Kanamycin resistant plants of Medicago varia A2 were obtained by an optimized procedure for high frequency transformation using Agrobacterium tumefaciens infection of leaf and petiole tissue. Parameters which affected the frequency were explant type, the Agrobacterium strain used and the time allowed for cocultivation. Under optimum conditions, i.e., using the Agrobacterium strain A281 and a 4 day cocultivation period, the frequency of transformed leaflets obtained was greater than 70%.

Entities:  

Year:  1988        PMID： 24240404     DOI： 10.1007/BF00272744

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  6 in total

1.  Aminoglycoside-modifying enzymes.

Authors:  M J Haas; J E Dowding
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

2.  Virulence of Agrobacterium tumefaciens Strain A281 on Legumes.

Authors:  E E Hood; R T Fraley; M D Chilton
Journal:  Plant Physiol       Date:  1987-03       Impact factor: 8.340

3.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Authors:  D A Melton; P A Krieg; M R Rebagliati; T Maniatis; K Zinn; M R Green
Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

4.  Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.

Authors:  G Ditta; S Stanfield; D Corbin; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205

5.  Transformation of Medicago by Agrobacterium mediated gene transfer.

Authors:  M Deak; G B Kiss; C Koncz; D Dudits
Journal:  Plant Cell Rep       Date:  1986-04       Impact factor: 4.570

6.  New cloning vehicles for transformation of higher plants.

Authors:  G An; B D Watson; S Stachel; M P Gordon; E W Nester
Journal:  EMBO J       Date:  1985-02       Impact factor: 11.598
  6 in total
  15 in total

1.  Efficient transformation of Medicago truncatula cv. Jemalong using the hypervirulent Agrobacterium tumefaciens strain AGL1.

Authors:  M Chabaud; F de Carvalho-Niebel; D G Barker
Journal:  Plant Cell Rep       Date:  2003-06-24       Impact factor: 4.570

2.  Constitutive expression of the beta-phaseolin gene in different tissues of transgenic alfalfa does not ensure phaseolin accumulation in non-seed tissue.

Authors:  S Bagga; D Sutton; J D Kemp; C Sengupta-Gopalan
Journal:  Plant Mol Biol       Date:  1992-09       Impact factor: 4.076

3.  Stress responses in alfalfa (Medicago sativa L.) 12. Sequence analysis of phenylalanine ammonia-lyase (PAL) cDNA clones and appearance of PAL transcripts in elicitor-treated cell cultures and developing plants.

Authors:  G Gowri; N L Paiva; R A Dixon
Journal:  Plant Mol Biol       Date:  1991-09       Impact factor: 4.076

4.  Stress responses in alfalfa (Medicago sativa L.). 8. Cis-elements and trans-acting factors for the quantitative expression of a bean chalcone synthase gene promoter in electroporated alfalfa protoplasts.

Authors:  M J Harrison; A D Choudhary; I Dubery; C J Lamb; R A Dixon
Journal:  Plant Mol Biol       Date:  1991-05       Impact factor: 4.076

5.  Effect of plant genotype on the transformation of cultivated alfalfa (Medicago sativa) by Agrobacterium tumefaciens.

Authors:  S Du; L Erickson; S Bowley
Journal:  Plant Cell Rep       Date:  1994-03       Impact factor: 4.570

6.  Agrobacterium tumefaciens-mediated beta-glucuronidase (GUS) gene expression in lentil (Lens culinaris Medik.) tissues.

Authors:  T D Warkentin; A McHughen
Journal:  Plant Cell Rep       Date:  1992-06       Impact factor: 4.570

7.  Optimizing the production of transformed pea (Pisum sativum L.) callus using disarmed Agrobacterium tumefaciens strains.

Authors:  M M Lulsdorf; H Rempel; J A Jackson; D S Baliski; S L Hobbs
Journal:  Plant Cell Rep       Date:  1991-01       Impact factor: 4.570

8.  Introduction and expression of an insect proteinase inhibitor in alfalfa Medicago sativa L.

Authors:  J C Thomas; C C Wasmann; C Echt; R L Dunn; H J Bohnert; T J McCoy
Journal:  Plant Cell Rep       Date:  1994-11       Impact factor: 4.570

9.  Agrobacterium-mediated transformation of apple (Malus x domestica Borkh.): an assessment of factors affecting gene transfer efficiency during early transformation steps.

Authors:  A De Bondt; K Eggermont; P Druart; M De Vil; I Goderis; J Vanderleyden; W F Broekaert
Journal:  Plant Cell Rep       Date:  1994-07       Impact factor: 4.570

10.  Stress responses in alfalfa (Medicago sativa L.) IV. Expression of defense gene constructs in electroporated suspension cell protoplasts.

Authors:  A D Choudhary; H Kessmann; C J Lamb; R A Dixon
Journal:  Plant Cell Rep       Date:  1990-06       Impact factor: 4.570
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