Literature DB >> 24186478

A high-frequency transformation system for the yeast Kluyveromyces lactis.

S Das1, C P Hollenberg.   

Abstract

This communication describes conditions for Kluyveromyces lactis protoplast regeneration and transformation and shows that a high frequency of transformation can be obtained with recombinant plasmids containing one of a series of K. lactis DNA fragments that presumably carry autonomously replicating sequences (called KARS). The vector YRp7, which contains a Saccharomyces cerevisiae autonomously replicating sequence (ARS), only led to integrative transformation of K. lactis indicating substantial differences in specificity between the DNA replication mechanisms of both yeast species. It is further shown that 2-μm DNA derived vectors giving high frequency transformation in S. cerevisiae can transform K. lactis only with low frequency.

Entities:  

Year:  1982        PMID： 24186478     DOI： 10.1007/BF00435211

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  15 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  High-frequency transformation of the fission yeast Schizosaccharomyces pombe.

Authors:  D Beach; P Nurse
Journal:  Nature       Date:  1981-03-12       Impact factor: 49.962

3.  Molecular cloning and expression in E. coli of a yeast gene coding for beta-galactosidase.

Authors:  R C Dickson; J S Markin
Journal:  Cell       Date:  1978-09       Impact factor: 41.582

4.  Synthesis of high molecular weight polypeptides in Escherichia coli minicells directed by cloned Saccharomyces cerevisiae 2-micron DNA.

Authors:  C P Hollenberg; B Kustermann-Kuhn; H D Royer
Journal:  Gene       Date:  1976       Impact factor: 3.688

5.  High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

Authors:  K Struhl; D T Stinchcomb; S Scherer; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

6.  Transformation of yeast by a replicating hybrid plasmid.

Authors:  J D Beggs
Journal:  Nature       Date:  1978-09-14       Impact factor: 49.962

7.  Expression of a transposable antibiotic resistance element in Saccharomyces.

Authors:  A Jimenez; J Davies
Journal:  Nature       Date:  1980-10-30       Impact factor: 49.962

8.  Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.

Authors:  G Tschumper; J Carbon
Journal:  Gene       Date:  1980-07       Impact factor: 3.688

9.  Mutations affecting synthesis of beta-galactosidase activity in the yeast Kluyveromyces lactis.

Authors:  R M Sheetz; R C Dickson
Journal:  Genetics       Date:  1980-08       Impact factor: 4.562

10.  Isolation of a yeast centromere and construction of functional small circular chromosomes.

Authors:  L Clarke; J Carbon
Journal:  Nature       Date:  1980-10-09       Impact factor: 49.962
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  19 in total

1.  Recruitment of the transcriptional machinery through GAL11P: structure and interactions of the GAL4 dimerization domain.

Authors:  P Hidalgo; A Z Ansari; P Schmidt; B Hare; N Simkovich; S Farrell; E J Shin; M Ptashne; G Wagner
Journal:  Genes Dev       Date:  2001-04-15       Impact factor: 11.361

2.  Centromeric DNA of Kluyveromyces lactis.

Authors:  J J Heus; B J Zonneveld; H Y Steensma; J A Van den Berg
Journal:  Curr Genet       Date:  1990-12       Impact factor: 3.886

3.  Cloning vectors of mitochondrial origin for eukaryotes: A new concept in genetic engineering.

Authors:  K Esser; U Kück; U Stahl; P Tudzynski
Journal:  Curr Genet       Date:  1983-07       Impact factor: 3.886

4.  Functional relationship among TATA sequences, gene induction and transcription initiation in the beta-galactosidase, LAC4, gene from Kluyveromyces lactis.

Authors:  A G Ficca; C P Hollenberg
Journal:  Curr Genet       Date:  1989-04       Impact factor: 3.886

5.  Distribution of mitochondrial intron sequences among 21 yeast species.

Authors:  P J Skelly; R Maleszka
Journal:  Curr Genet       Date:  1991-02       Impact factor: 3.886

6.  Expression of an endogenous and a heterologous gene in Candida maltosa by using a promoter of a newly-isolated phosphoglycerate kinase (PGK) gene.

Authors:  Y Masuda; S M Park; M Ohkuma; A Ohta; M Takagi
Journal:  Curr Genet       Date:  1994-05       Impact factor: 3.886

7.  A mobile group II intron of a naturally occurring rearranged mitochondrial genome in Kluyveromyces lactis.

Authors:  P J Skelly; C M Hardy; G D Clark-Walker
Journal:  Curr Genet       Date:  1991-07       Impact factor: 3.886

8.  Multiple-copy integration of the alpha-galactosidase gene from Cyamopsis tetragonoloba into the ribosomal DNA of Kluyveromyces lactis.

Authors:  R J Bergkamp; I M Kool; R H Geerse; R J Planta
Journal:  Curr Genet       Date:  1992-04       Impact factor: 3.886

9.  Generation of a stable non-reverting Leu- mutant of Kluyveromyces lactis by gene disruption.

Authors:  F Meinhardt; R Holtwick
Journal:  Appl Microbiol Biotechnol       Date:  1995-01       Impact factor: 4.813

10.  Centromere promoter factors (CPF1) of the yeasts Saccharomyces cerevisiae and Kluyveromyces lactis are functionally exchangeable, despite low overall homology.

Authors:  W Mulder; A A Winkler; I H Scholten; B J Zonneveld; J H de Winde; H Yde Steensma; L A Grivell
Journal:  Curr Genet       Date:  1994-09       Impact factor: 3.886
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