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Literature DB >> 24186369

r-DNA plasmid from Schizosaccharomyces pombe: Cloning and use in yeast transformation.

P Fournier¹, C Gaillardin, L de Louvencourt, H Heslot, B F Lang, F Kaudewitz.

Abstract

1. an extrachromosomal, extramitochondrial DNA circle of about 3 μm length, occurring in S. pombe, was cloned in pBR322 and a detailed restriction map of the cloned sequence constructed. 2. Hybridization of this DNA with the ribosomal DNA from S. cerevisiae revealed regions of homology which were mapped in the cloned S. pombe DNA. 3. A transformation system was developed using the URA3 gene of S. cerevisiae and the 3 μm molecule. It was shown that the r-DNA part of the hybrid plasmid promotes replication in S. pombe.

Entities: Gene Species

Year: 1982 PMID： 24186369 DOI： 10.1007/BF00397639

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

16 in total

1. Methodological Information on the Genetics of Schizosaccharomyces Pombe.

Authors: U LEUPOLD
Journal: Schweiz Z Pathol Bakteriol Date: 1955

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

4. High-frequency transformation of the fission yeast Schizosaccharomyces pombe.

Authors: D Beach; P Nurse
Journal: Nature Date: 1981-03-12 Impact factor: 49.962

5. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors: D B Clewell
Journal: J Bacteriol Date: 1972-05 Impact factor: 3.490

6. Qualitative detection of penicillinase produced by yeast strains carrying chimeric yeast-coli plasmids.

Authors: M R Chevallier; M Aigle
Journal: FEBS Lett Date: 1979-12-01 Impact factor: 4.124

7. 2 micrometer covalently closed non-mitochondrial circular DNA in the petite-negative yeast Schizosaccharomyces pombe.

Authors: L Del Giudice; K Wolf; P Sassone-Corsi; A Mazza
Journal: Mol Gen Genet Date: 1979-05-04

8. Nucleotide sequence of the yeast plasmid.

Authors: J L Hartley; J E Donelson
Journal: Nature Date: 1980-08-28 Impact factor: 49.962

9. Curing of Saccharomyces cerevisiae 2-μm DNA by transformation.

Authors: E Erhart; C P Hollenberg
Journal: Curr Genet Date: 1981-05 Impact factor: 3.886

10. Hybridizable sequences between cytoplasmic ribosomal RNAs and 3 micron circular DNAs of Saccharomyces cerevisiae and Torulopsis glabrata.

Authors: G D Clark-Walker; A A Azad
Journal: Nucleic Acids Res Date: 1980-03-11 Impact factor: 16.971

8 in total

6. Multiple orientation-dependent, synergistically interacting, similar domains in the ribosomal DNA replication origin of the fission yeast, Schizosaccharomyces pombe.

Authors: S M Kim; J A Huberman
Journal: Mol Cell Biol Date: 1998-12 Impact factor: 4.272

7. Transformation of Candida maltosa and Pichia guilliermondii by a plasmid containing Saccharomyces cerevisiae ARG4 DNA.

Authors: G Kunze; C Petzoldt; R Bode; I Samsonova; M Hecker; D Birnbaum
Journal: Curr Genet Date: 1985 Impact factor: 3.886

8. Rapid alkaline preparation for yeast circular covalently closed DNA molecules.

Authors: P Filetici; N Junakovic; P Ballario
Journal: Curr Genet Date: 1985 Impact factor: 3.886