Literature DB >> 24185797

Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts.

M Salmenkallio-Marttila1, K Aspegren, S Akerman, U Kurtén, L Mannonen, A Ritala, T H Teeri, V Kauppinen.   

Abstract

Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation. Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals. Southern blot analysis and NPTII assays of second and third generation plants confirmed the genomic integration of the transferred gene and that the new trait was inherited by the progeny.

Entities:  

Year:  1995        PMID： 24185797     DOI： 10.1007/BF00193741

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  12 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Transgenic rice plants produced by electroporation-mediated plasmid uptake into protoplasts.

Authors:  H M Zhang; H Yang; E L Rech; T J Golds; A S Davis; B J Mulligan; E C Cocking; M R Davey
Journal:  Plant Cell Rep       Date:  1988-10       Impact factor: 4.570

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase.

Authors:  G Van den Broeck; M P Timko; A P Kausch; A R Cashmore; M Van Montagu; L Herrera-Estrella
Journal:  Nature       Date:  1985 Jan 31-Feb 6       Impact factor: 49.962

5.  Genetically transformed maize plants from protoplasts.

Authors:  C A Rhodes; D A Pierce; I J Mettler; D Mascarenhas; J J Detmer
Journal:  Science       Date:  1988-04-08       Impact factor: 47.728

6.  A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extracts.

Authors:  B Reiss; R Sprengel; H Will; H Schaller
Journal:  Gene       Date:  1984-10       Impact factor: 3.688

7.  Efficient regeneration of transgenic plants from rice protoplasts and correctly regulated expression of the foreign gene in the plants.

Authors:  W Zhang; R Wu
Journal:  Theor Appl Genet       Date:  1988-12       Impact factor: 5.699

8.  Efficient regeneration of fertile plants from protoplasts isolated from microspore cultures of barley (Hordeum vulgare L.).

Authors:  M Salmenkallio-Marttila; V Kauppinen
Journal:  Plant Cell Rep       Date:  1995-01       Impact factor: 4.570

9.  Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts.

Authors:  P A Lazzeri; R Brettschneider; R Lührs; H Lörz
Journal:  Theor Appl Genet       Date:  1991-04       Impact factor: 5.699

10.  Regeneration of fertile plants from protoplasts derived from embryogenic cell suspensions of barley (Hordeum vulgare L.).

Authors:  A Jähne; P A Lazzeri; H Lörz
Journal:  Plant Cell Rep       Date:  1991-05       Impact factor: 4.570
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  3 in total

1.  Expression of fungal thermotolerant endo-1,4-beta-glucanase in transgenic barley seeds during germination.

Authors:  A M Nuutila; A Ritala; R W Skadsen; L Mannonen; V Kauppinen
Journal:  Plant Mol Biol       Date:  1999-12       Impact factor: 4.076

Review 2.  Gene transfer to plants by electroporation: methods and applications.

Authors:  Ibrahim Ilker Ozyigit
Journal:  Mol Biol Rep       Date:  2020-04-02       Impact factor: 2.316

3.  Control of nuclear and nucleolar localization of nuclear inclusion protein a of picorna-like Potato virus A in Nicotiana species.

Authors:  Minna-Liisa Rajamäki; Jari P T Valkonen
Journal:  Plant Cell       Date:  2009-08-21       Impact factor: 11.277
  3 in total

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