Literature DB >> 24178464

A reliable method for extraction of RNA from various conifer tissues.

J Z Dong1, D I Dunstan.   

Abstract

A simple and efficient procedure suitable for extraction of high-quality RNA from cultured conifer tissues, somatic embryos, zygotic embryos, needles, stem and root tissues was developed. It produced from 100 μg up to 700 μg total RNA per gram tissue dependent on the types of tissues used. RNA quality was estimated by spectrophotometry, agarose gel electrophoresis, in vitro translation of mRNA, cDNA synthesis and Northern blot analysis. The method also worked well with Arabidopsis thaliana and tobacco tissues.

Entities:  

Year:  1996        PMID： 24178464     DOI： 10.1007/BF00232985

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  11 in total

1.  An efficient method for isolation of RNA and DNA from plants containing polyphenolics.

Authors:  M E John
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

2.  Phenol as grinding material in RNA preparations.

Authors:  M Maes; E Messens
Journal:  Nucleic Acids Res       Date:  1992-08-25       Impact factor: 16.971

3.  RNA and DNA isolation from recalcitrant plant tissues.

Authors:  S S Baker; C L Rugh; J C Kamalay
Journal:  Biotechniques       Date:  1990-09       Impact factor: 1.993

4.  An efficient method for isolation of RNA from tissue cultured plant cells.

Authors:  M Shirzadegan; P Christie; J R Seemann
Journal:  Nucleic Acids Res       Date:  1991-11-11       Impact factor: 16.971

5.  A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis.

Authors:  G J Wadsworth; M G Redinbaugh; J G Scandalios
Journal:  Anal Biochem       Date:  1988-07       Impact factor: 3.365

6.  Improved method for the isolation of RNA from plant tissues.

Authors:  J Logemann; J Schell; L Willmitzer
Journal:  Anal Biochem       Date:  1987-05-15       Impact factor: 3.365

7.  Midiprep method for isolation of DNA from plants with a high content of polyphenolics.

Authors:  U Pich; I Schubert
Journal:  Nucleic Acids Res       Date:  1993-07-11       Impact factor: 16.971

8.  Northern hybridization: rapid and simple electrophoretic conditions.

Authors:  R Pellé; N B Murphy
Journal:  Nucleic Acids Res       Date:  1993-06-11       Impact factor: 16.971

9.  Transformation of white spruce (Picea glauca) somatic embryos by microprojectile bombardment.

Authors:  V R Bommineni; R N Chibbar; R S Datla; E W Tsang
Journal:  Plant Cell Rep       Date:  1993-11       Impact factor: 4.570

10.  GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

Authors:  R A Jefferson; T A Kavanagh; M W Bevan
Journal:  EMBO J       Date:  1987-12-20       Impact factor: 11.598
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  9 in total

1.  Expression of abundant mRNAs during somatic embryogenesis of white spruce [Picea glauca (Moench) Voss].

Authors:  J Z Dong; D I Dunstan
Journal:  Planta       Date:  1996       Impact factor: 4.116

2.  Cloning and characterization of six embryogenesis-associated cDNAs from somatic embryos of Picea glauca and their comparative expression during zygotic embryogenesis.

Authors:  J Z Dong; D I Dunstan
Journal:  Plant Mol Biol       Date:  1999-03       Impact factor: 4.076

3.  Soluble invertase expression is an early target of drought stress during the critical, abortion-sensitive phase of young ovary development in maize.

Authors:  Mathias Neumann Andersen; Folkard Asch; Yong Wu; Christian Richardt Jensen; Henrik Naested; Vagn Overgaard Mogensen; Karen Elaine Koch
Journal:  Plant Physiol       Date:  2002-10       Impact factor: 8.340

4.  Endochitinase and beta-1,3-glucanase genes are developmentally regulated during somatic embryogenesis in Picea glauca.

Authors:  J Z Dong; D I Dunstan
Journal:  Planta       Date:  1997       Impact factor: 4.116

5.  Gene expression at early stages of Brassica napus seed development as revealed by transcript profiling of seed-abundant cDNAs.

Authors:  Jinzhuo Dong; Wilf A Keller; Wei Yan; Fawzy Georges
Journal:  Planta       Date:  2003-10-23       Impact factor: 4.116

6.  A role for jasmonate in pathogen defense of Arabidopsis.

Authors:  P Vijayan; J Shockey; C A Lévesque; R J Cook; J Browse
Journal:  Proc Natl Acad Sci U S A       Date:  1998-06-09       Impact factor: 11.205

7.  Ginger and turmeric expressed sequence tags identify signature genes for rhizome identity and development and the biosynthesis of curcuminoids, gingerols and terpenoids.

Authors:  Hyun Jo Koo; Eric T McDowell; Xiaoqiang Ma; Kevin A Greer; Jeremy Kapteyn; Zhengzhi Xie; Anne Descour; HyeRan Kim; Yeisoo Yu; David Kudrna; Rod A Wing; Carol A Soderlund; David R Gang
Journal:  BMC Plant Biol       Date:  2013-02-15       Impact factor: 4.215

8.  Comparison of RNA isolation methods from insect larvae.

Authors:  J A Ridgeway; A E Timm
Journal:  J Insect Sci       Date:  2014-01-01       Impact factor: 1.857

9.  Optimization and comparison of different methods for RNA isolation for cDNA library construction from the reindeer lichen Cladonia rangiferina.

Authors:  Sini Junttila; Kean-Jin Lim; Stephen Rudd
Journal:  BMC Res Notes       Date:  2009-10-05
  9 in total

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