Literature DB >> 24177971

Outer envelope membranes from chloroplasts are isolated as right-side-out vesicles.

K Waegemann1, S Eichacker, J Soll.   

Abstract

Outer envelope membranes were isolated from purified chloroplasts of pea leaves. The sidedness of the vesicles was analyzed by (i) aqueous polymer-two phase partitioning, (ii) the effect of limited proteolysis on the outer-envelope proteins (OEP) 86 and OEP 7 in intact organelles and isolated membranes, (iii) fluorescence-microscopy and finally (iv) binding of precursor polypeptides to isolated outer-membrane vesicles. The results demonstrate that purified outer envelope membranes occur largely (>90%) as right-side-out vesicles.

Year:  1992        PMID： 24177971     DOI： 10.1007/BF00201628

Source DB:  PubMed          Journal:  Planta        ISSN： 0032-0935            Impact factor:   4.116


  16 in total

1.  COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

Authors:  D I Arnon
Journal:  Plant Physiol       Date:  1949-01       Impact factor: 8.340

2.  Thermolysin is a suitable protease for probing the surface of intact pea chloroplasts.

Authors:  K Cline; M Werner-Washburne; J Andrews; K Keegstra
Journal:  Plant Physiol       Date:  1984-07       Impact factor: 8.340

3.  Sequence analysis and protein import studies of an outer chloroplast envelope polypeptide.

Authors:  M Salomon; K Fischer; U I Flügge; J Soll
Journal:  Proc Natl Acad Sci U S A       Date:  1990-08       Impact factor: 11.205

4.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 7.  Phase partition--a method for purification and analysis of cell organelles and membrane vesicles.

Authors:  P A Albertsson; B Andersson; C Larsson; H E Akerlund
Journal:  Methods Biochem Anal       Date:  1982

8.  Localization of polypeptides to the cytosolic side of the outer envelope membrane of spinach chloroplasts.

Authors:  J Joyard; A Billecocq; S G Bartlett; M A Block; N H Chua; R Douce
Journal:  J Biol Chem       Date:  1983-08-25       Impact factor: 5.157

9.  Separation and characterization of inner and outer envelope membranes of pea chloroplasts.

Authors:  K Cline; J Andrews; B Mersey; E H Newcomb; K Keegstra
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205

10.  Adenylate effects on protein phosphorylation in the interenvelope lumen of pea chloroplasts.

Authors:  J Soll; V Berger; J Bennett
Journal:  Planta       Date:  1989-03       Impact factor: 4.116
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  15 in total

1.  Toc12, a novel subunit of the intermembrane space preprotein translocon of chloroplasts.

Authors:  Thomas Becker; Jozef Hritz; Markus Vogel; Alexander Caliebe; Bernd Bukau; Jürgen Soll; Enrico Schleiff
Journal:  Mol Biol Cell       Date:  2004-08-18       Impact factor: 4.138

Review 2.  Protein translocation into and across the chloroplastic envelope membranes.

Authors:  J Soll; R Tien
Journal:  Plant Mol Biol       Date:  1998-09       Impact factor: 4.076

3.  Isolation and characterization of an amino acid-selective channel protein present in the chloroplastic outer envelope membrane.

Authors:  K Pohlmeyer; J Soll; T Steinkamp; S Hinnah; R Wagner
Journal:  Proc Natl Acad Sci U S A       Date:  1997-08-19       Impact factor: 11.205

4.  Lipid composition of outer leaflet of chloroplast outer envelope determines topology of OEP7.

Authors:  E Schleiff; R Tien; M Salomon; J Soll
Journal:  Mol Biol Cell       Date:  2001-12       Impact factor: 4.138

5.  Chloroplast Omp85 proteins change orientation during evolution.

Authors:  Maik S Sommer; Bertram Daum; Lucia E Gross; Benjamin L M Weis; Oliver Mirus; Lars Abram; Uwe-G Maier; Werner Kühlbrandt; Enrico Schleiff
Journal:  Proc Natl Acad Sci U S A       Date:  2011-08-08       Impact factor: 11.205

6.  Plant-Specific Preprotein and Amino Acid Transporter Proteins Are Required for tRNA Import into Mitochondria.

Authors:  Monika W Murcha; Szymon Kubiszewski-Jakubiak; Pedro F Teixeira; Irene L Gügel; Beata Kmiec; Reena Narsai; Aneta Ivanova; Cyrille Megel; Annette Schock; Sabrina Kraus; Oliver Berkowitz; Elzbieta Glaser; Katrin Philippar; Laurence Maréchal-Drouard; Jürgen Soll; James Whelan
Journal:  Plant Physiol       Date:  2016-10-27       Impact factor: 8.340

7.  OEP40, a Regulated Glucose-permeable β-Barrel Solute Channel in the Chloroplast Outer Envelope Membrane.

Authors:  Anke Harsman; Annette Schock; Birgit Hemmis; Vanessa Wahl; Ingrid Jeshen; Philipp Bartsch; Armin Schlereth; Heidi Pertl-Obermeyer; Tom Alexander Goetze; Jürgen Soll; Katrin Philippar; Richard Wagner
Journal:  J Biol Chem       Date:  2016-06-23       Impact factor: 5.157

8.  The plastid outer envelope protein OEP16 affects metabolic fluxes during ABA-controlled seed development and germination.

Authors:  Birgit Pudelski; Annette Schock; Stefan Hoth; Ruslana Radchuk; Hans Weber; Jörg Hofmann; Uwe Sonnewald; Jürgen Soll; Katrin Philippar
Journal:  J Exp Bot       Date:  2011-12-09       Impact factor: 6.992

9.  Toc64, a new component of the protein translocon of chloroplasts.

Authors:  K Sohrt; J Soll
Journal:  J Cell Biol       Date:  2000-03-20       Impact factor: 10.539

10.  Characterization of the translocon of the outer envelope of chloroplasts.

Authors:  Enrico Schleiff; Jürgen Soll; Michael Küchler; Werner Kühlbrandt; Roswitha Harrer
Journal:  J Cell Biol       Date:  2003-02-17       Impact factor: 10.539
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