Literature DB >> 16593034

Separation and characterization of inner and outer envelope membranes of pea chloroplasts.

K Cline1, J Andrews, B Mersey, E H Newcomb, K Keegstra.   

Abstract

A procedure for purifying the chloroplast envelope subfractionates it into two membrane fractions of comparable quantities. This procedure differs from previous ones in that the chloroplasts are ruptured by freezing and thawing in hypertonic medium rather than by osmotic shock. The two membrane fractions have qualitatively similar polar lipid compositions but differ in their content of individual lipids, specifically monogalactosyldiacylglycerol and phosphatidylcholine. The two fractions also differ in their constituent polypeptides and in their appearance when examined by electron microscopy. The light (density = 1.08 g/ml) and heavy (density = 1.13 g/ml) membrane fractions have been tentatively identified as the outer and inner envelope membranes, respectively.

Entities:  

Year:  1981        PMID： 16593034      PMCID： PMC319617          DOI： 10.1073/pnas.78.6.3595

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  10 in total

1.  [A SIMPLE METHOD FOR MICRODETERMINATION OF PHOSPHATE IN PAPER CHROMATOGRAPHY].

Authors:  E GERLACH; B DEUTICKE
Journal:  Biochem Z       Date:  1963-07-26

2.  COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

Authors:  D I Arnon
Journal:  Plant Physiol       Date:  1949-01       Impact factor: 8.340

3.  A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.

Authors:  M A Markwell; S M Haas; L L Bieber; N E Tolbert
Journal:  Anal Biochem       Date:  1978-06-15       Impact factor: 3.365

4.  A low-viscosity epoxy resin embedding medium for electron microscopy.

Authors:  A R Spurr
Journal:  J Ultrastruct Res       Date:  1969-01

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  The inner membrane of the chloroplast envelope as the site of specific metabolite transport.

Authors:  H W Heldt; F Sauer
Journal:  Biochim Biophys Acta       Date:  1971-04-06

7.  Ultrastructural and biochemical studies of mitoplasts and outer membranes derived from French-pressed mitochondria. Advances in mitochondrial subfractionation.

Authors:  G L Decker; J W Greenawalt
Journal:  J Ultrastruct Res       Date:  1977-04

8.  The external NADH dehydrogenases of intact plant mitochondria.

Authors:  R Douce; C A Mannella; W D Bonner
Journal:  Biochim Biophys Acta       Date:  1973-01-18

9.  An improved method for the isolation of spinach chloroplast envelope membranes.

Authors:  R P Poincelot; P R Day
Journal:  Plant Physiol       Date:  1974-11       Impact factor: 8.340

Review 10.  Transport of proteins into mitochondria and chloroplasts.

Authors:  N H Chua; G W Schmidt
Journal:  J Cell Biol       Date:  1979-06       Impact factor: 10.539
  10 in total
  65 in total

Review 1.  News in chloroplast protein import.

Authors:  A Caliebe; J Soill
Journal:  Plant Mol Biol       Date:  1999-03       Impact factor: 4.076

2.  The predicted candidates of Arabidopsis plastid inner envelope membrane proteins and their expression profiles.

Authors:  Abraham J K Koo; John B Ohlrogge
Journal:  Plant Physiol       Date:  2002-10       Impact factor: 8.340

3.  Proteomics of chloroplast envelope membranes.

Authors:  Norbert Rolland; Myriam Ferro; Daphné Seigneurin-Berny; Jérôme Garin; Roland Douce; Jacques Joyard
Journal:  Photosynth Res       Date:  2003       Impact factor: 3.573

4.  Production, characterization, and applications of monoclonal antibodies reactive with soybean nodule xanthine dehydrogenase.

Authors:  E W Triplett; C R Lending; D J Gumpf; C F Ware
Journal:  Plant Physiol       Date:  1986-04       Impact factor: 8.340

5.  Respiration of Sugars in Spinach (Spinacia oleracea), Maize (Zea mays), and Chlamydomonas reinhardtii F-60 Chloroplasts with Emphasis on the Hexose Kinases.

Authors:  K. K. Singh; C. Chen; D. K. Epstein; M. Gibbs
Journal:  Plant Physiol       Date:  1993-06       Impact factor: 8.340

6.  In situ incorporation of Fatty acids into lipids of the outer and inner envelope membranes of pea chloroplasts.

Authors:  M Miquel; J P Dubacq
Journal:  Plant Physiol       Date:  1992-09       Impact factor: 8.340

7.  Adenylate effects on protein phosphorylation in the interenvelope lumen of pea chloroplasts.

Authors:  J Soll; V Berger; J Bennett
Journal:  Planta       Date:  1989-03       Impact factor: 4.116

8.  Do thylakoids really contain phosphatidylcholine?

Authors:  A J Dorne; J Joyard; R Douce
Journal:  Proc Natl Acad Sci U S A       Date:  1990-01       Impact factor: 11.205

9.  Subcellular localization of triacylglycerol synthesis in spinach leaves.

Authors:  B A Martin; R F Wilson
Journal:  Lipids       Date:  1984-02       Impact factor: 1.880

10.  Mode of action of the COR15a gene on the freezing tolerance of Arabidopsis thaliana.

Authors:  P L Steponkus; M Uemura; R A Joseph; S J Gilmour; M F Thomashow
Journal:  Proc Natl Acad Sci U S A       Date:  1998-11-24       Impact factor: 11.205
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