Linkage information content and efficiency of full-sib and half-sib designs for gene mapping.

The accuracy of a genetic map depends on the amount of linkage information contained in the data set used for construction of the map. The amount of linkage information is related to the designs employed for linkage analysis. The purpose of this study was to provide general formulations for various genotyping schemes and family structures in order to evaluate the amount of linkage information in a data set. Linkage information content (LIC) was defined as the frequency of fully informative gametes, which are gametes from doubly heterozygous parents with known linkage phases. Depending on the design, LIC is based on two generations if the parental phases are determined statistically, or three generations if the parental phases are determined genetically. Different schemes were considered in deriving LIC: (1) genotyping of one parent or two parents, and (2) genotyping of two or three generation families. The LIC for a full-sib design was found to be generally greater than for a half-sib design but requires typing a large number of individuals when at least one locus has only two alleles. The efficiency of the full-sib design is reduced significantly if a sex-specific linkage map is sought.