Literature DB >> 24173448

Analysis of yeast DNA by alkaline filter elution.

J Zuk1, D Zaborowska, Z Swietlińska.   

Abstract

The method of analysis of DNA in mammalian cells by alkaline elution from filters (Kohn et al. 1974) was adapted for studies on yeast DNA. By this technique spheroplasts obtained from yeast cells are lysed on filters and single-stranded DNA fragments selectively eluted by alkaline solutions. The procedure was applied to monitor the occurrence of replication intermediates and production of DNA single-strand breakage by MMS, and its repair in growth medium.

Entities:  

Year:  1983        PMID： 24173448     DOI： 10.1007/BF00377607

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  11 in total

1.  Fractionation of DNA from mammalian cells by alkaline elution.

Authors:  K W Kohn; L C Erickson; R A Ewig; C A Friedman
Journal:  Biochemistry       Date:  1976-10-19       Impact factor: 3.162

2.  Fiber autoradiography of replicating yeast DNA.

Authors:  T D Petes; D H Williamson
Journal:  Exp Cell Res       Date:  1975-10-01       Impact factor: 3.905

3.  Alkaline sucrose sedimentation studies of MMS-induced DNA single-strand breakage and rejoining in the wild type and in UV-sensitive mutants of Saccharomyces cerevisiae.

Authors:  W J Jachymczyk; E Chlebowicz; Z Swietlinska; J Zuk
Journal:  Mutat Res       Date:  1977-04       Impact factor: 2.433

4.  Repair of MMS-induced DNA double-strand breaks in haploid cells of Saccharomyces cerevisiae, which requires the presence of a duplicate genome.

Authors:  E Chlebowicz; W J Jachymczyk
Journal:  Mol Gen Genet       Date:  1979-01-02

5.  Structure of DNA in DNA replication mutants of yeast.

Authors:  T D Petes; C S Newlon
Journal:  Nature       Date:  1974-10-18       Impact factor: 49.962

6.  DNA labelling and its assay in yeast.

Authors:  J Hatzfeld
Journal:  Biochim Biophys Acta       Date:  1973-02-23

7.  DNA chain growth during replication of asynchronous L1210 cells. Alkaline elution of large DNA segments from cells lysed on filters.

Authors:  K W Kohn; C A Friedman; R A Ewig; Z M Iqbal
Journal:  Biochemistry       Date:  1974-09-24       Impact factor: 3.162

8.  An alkaline sucrose gradient analysis of the mechanism of nuclear DNA synthesis in the yeast Saccharomyces cerevisiae.

Authors:  L H Johnston; D H Williamson
Journal:  Mol Gen Genet       Date:  1978-08-17

9.  Isolation and purification of large quantities of DNA replication intermediates by pH step alkaline elution.

Authors:  L C Erickson; W E Ross; K W Kohn
Journal:  Chromosoma       Date:  1979       Impact factor: 4.316

10.  Replication fork rate and origin activation during the S phase of Saccharomyces cerevisiae.

Authors:  C J Rivin; W L Fangman
Journal:  J Cell Biol       Date:  1980-04       Impact factor: 10.539
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  1 in total

1.  Repair of 8-methoxypsoralen photoinduced cross-links in yeast. Analysis by alkaline step-elution and electron microscopy.

Authors:  E Cundari; M Dardalhon; S Rousset; D Averbeck
Journal:  Mol Gen Genet       Date:  1991-09
  1 in total

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