Literature DB >> 24170959

A method to measure cellular adhesion utilizing a polymer micro-cantilever.

Angelo Gaitas1, Ricky Malhotra, Kenneth Pienta.   

Abstract

In the present study we engineered a micro-machined polyimide cantilever with an embedded sensing element to investigate cellular adhesion, in terms of its relative ability to stick to a cross-linker, 3,3'-dithiobis[sulfosuccinimidylpropionate], coated on the cantilever surface. To achieve this objective, we investigated adhesive properties of three human prostate cancer cell lines, namely, a bone metastasis derived human prostate cancer cell line (PC3), a brain metastasis derived human prostate cancer cell line (DU145), and a subclone of PC3 (PC3-EMT14). We found that PC3-EMT14, which displays a mesenchymal phenotype, has the least adhesion compared to PC3 and DU145, which exhibit an epithelial phenotype.

Entities:  

Year:  2013        PMID： 24170959      PMCID： PMC3790771          DOI： 10.1063/1.4821946

Source DB:  PubMed          Journal:  Appl Phys Lett        ISSN： 0003-6951            Impact factor:   3.791


  17 in total

1.  Cross-linking of cell surface receptors enhances cooperativity of molecular adhesion.

Authors:  A Chen; V T Moy
Journal:  Biophys J       Date:  2000-06       Impact factor: 4.033

2.  Discrete interactions in cell adhesion measured by single-molecule force spectroscopy.

Authors:  M Benoit; D Gabriel; G Gerisch; H E Gaub
Journal:  Nat Cell Biol       Date:  2000-06       Impact factor: 28.824

3.  Measuring cell adhesion forces with the atomic force microscope at the molecular level.

Authors:  Martin Benoit; Hermann E Gaub
Journal:  Cells Tissues Organs       Date:  2002       Impact factor: 2.481

4.  Determination of cellular mechanical properties by cell poking, with an application to leukocytes.

Authors:  G I Zahalak; W B McConnaughey; E L Elson
Journal:  J Biomech Eng       Date:  1990-08       Impact factor: 2.097

Review 5.  Regulation of cadherin-mediated adhesion in morphogenesis.

Authors:  Barry M Gumbiner
Journal:  Nat Rev Mol Cell Biol       Date:  2005-08       Impact factor: 94.444

Review 6.  NF-kappaB and epithelial to mesenchymal transition of cancer.

Authors:  Chengyin Min; Sean F Eddy; David H Sherr; Gail E Sonenshein
Journal:  J Cell Biochem       Date:  2008-06-01       Impact factor: 4.429

Review 7.  The basics of epithelial-mesenchymal transition.

Authors:  Raghu Kalluri; Robert A Weinberg
Journal:  J Clin Invest       Date:  2009-06       Impact factor: 14.808

Review 8.  The role of Notch signaling pathway in epithelial-mesenchymal transition (EMT) during development and tumor aggressiveness.

Authors:  Zhiwei Wang; Yiwei Li; Dejuan Kong; Fazlul H Sarkar
Journal:  Curr Drug Targets       Date:  2010-06       Impact factor: 3.465

Review 9.  The epithelial-mesenchymal transition: new insights in signaling, development, and disease.

Authors:  Jonathan M Lee; Shoukat Dedhar; Raghu Kalluri; Erik W Thompson
Journal:  J Cell Biol       Date:  2006-03-27       Impact factor: 10.539

10.  Epithelial to mesenchymal transition of a primary prostate cell line with switches of cell adhesion modules but without malignant transformation.

Authors:  Xi-Song Ke; Yi Qu; Naomi Goldfinger; Kari Rostad; Randi Hovland; Lars A Akslen; Varda Rotter; Anne Margrete Øyan; Karl-Henning Kalland
Journal:  PLoS One       Date:  2008-10-13       Impact factor: 3.240
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  4 in total

1.  A device for rapid and quantitative measurement of cardiac myocyte contractility.

Authors:  Angelo Gaitas; Ricky Malhotra; Tao Li; Todd Herron; José Jalife
Journal:  Rev Sci Instrum       Date:  2015-03       Impact factor: 1.523

Review 2.  Extending applications of AFM to fluidic AFM in single living cell studies.

Authors:  Yuan Qiu; Chen-Chi Chien; Basile Maroulis; Jiani Bei; Angelo Gaitas; Bin Gong
Journal:  J Cell Physiol       Date:  2022-06-13       Impact factor: 6.513

3.  A micromachined force sensing apparatus and method for human engineered cardiac tissue and induced pluripotent stem cell characterization.

Authors:  Irene C Turnbull; Weibin Zhu; Francesca Stillitano; Chen-Chi Chien; Angelo Gaitas
Journal:  Sens Actuators A Phys       Date:  2021-06-01       Impact factor: 4.291

4.  Real-time Detection of Breast Cancer Cells Using Peptide-functionalized Microcantilever Arrays.

Authors:  Hashem Etayash; Keren Jiang; Sarfuddin Azmi; Thomas Thundat; Kamaljit Kaur
Journal:  Sci Rep       Date:  2015-10-05       Impact factor: 4.379
  4 in total

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