Literature DB >> 2415922

Selective protection of in vitro synthesized cDNA against nucleases by incorporation of phosphorothioate-analogues.

G Schreiber, E M Koch, W J Neubert.   

Abstract

The conditions for the stepwise synthesis of single- (ss) and double-stranded (ds) cDNA using thio-analogues instead of dNTPs are described in this paper. RNA of paramyxovirus Sendai (strain 6/94) serves as template in these experiments. The increased resistance of this alpha S-modified cDNA against several nucleases, like S1-Nuclease, DNase I, Exonuclease III, snake venom Phosphodiesterase (PDE) and the combination of DNase I and PDE is demonstrated.

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Year:  1985        PMID： 2415922      PMCID： PMC322078          DOI： 10.1093/nar/13.21.7663

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Studies on polynucleotides. III. Enzymic degradation; substrate specificity and properties of snake venom phosphodiesterase.

Authors:  W E RAZZELL; H G KHORANA
Journal:  J Biol Chem       Date:  1959-08       Impact factor: 5.157

2.  Endonuclease II of Escherichia coli is exonuclease III.

Authors:  B Weiss
Journal:  J Biol Chem       Date:  1976-04-10       Impact factor: 5.157

3.  Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.

Authors:  A E Sippel
Journal:  Eur J Biochem       Date:  1973-08-01

4.  Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.

Authors:  V M Vogt
Journal:  Eur J Biochem       Date:  1973-02-15

5.  Stereochemical course of polymerization catalyzed by avian myeloblastosis virus reverse transcriptase.

Authors:  P A Bartlett; F Eckstein
Journal:  J Biol Chem       Date:  1982-08-10       Impact factor: 5.157

6.  Purification and properties of S1 nuclease from Aspergillus.

Authors:  V M Vogt
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

7.  Incorporation of phosphorothioate groups into fd and phi X174 DNA.

Authors:  H P Vosberg; F Eckstein
Journal:  Biochemistry       Date:  1977-08-09       Impact factor: 3.162

8.  Effect of deoxynucleoside phosphorothioates incorporated in DNA on cleavage by restriction enzymes.

Authors:  H P Vosberg; F Eckstein
Journal:  J Biol Chem       Date:  1982-06-10       Impact factor: 5.157

9.  A DNA fragment with an alpha-phosphorothioate nucleotide at one end is asymmetrically blocked from digestion by exonuclease III and can be replicated in vivo.

Authors:  S D Putney; S J Benkovic; P R Schimmel
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

10.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Authors:  J M Chirgwin; A E Przybyla; R J MacDonald; W J Rutter
Journal:  Biochemistry       Date:  1979-11-27       Impact factor: 3.162
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  1 in total

1.  Inhibition of deoxyribonucleases by phosphorothioate groups in oligodeoxyribonucleotides.

Authors:  S Spitzer; F Eckstein
Journal:  Nucleic Acids Res       Date:  1988-12-23       Impact factor: 16.971
  1 in total

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