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Literature DB >> 24156043

Hyper-numerical aperture (NA = 2.8) microscope using λ = 1.56 µm femtosecond source for multi-photon imaging.

Youngsik Kim¹, Phat Lu, Tom D Milster, Khanh Kieu.

Abstract

A new microscope is discussed, where the scanning illumination has a numerical aperture of 2.8 with λ = 1.56 µm femtosecond fiber laser. Samples are placed or grown on a silicon substrate. Multi-photon emission is imaged in transmission on a cooled CCD. Two-photon and three-photon effects are observed from the silicon/water interface and gold nanoparticles. Images of cells, reference spheres and gold nanoparticles illustrate imaging properties of the microscope. Spectral characteristics of individual particles are achieved with a blazed transmission grating. Emission properties of differently sized gold nanoparticles are studied in detail, which indicate that their emission is a two-photon effect due continuum generation. Interestingly, spectral shape and emission power are similar for 20nm, 40nm and 60nm diameter gold nanoparticles for the cases studied.

Entities: Chemical

Keywords: (180.4243) Near-field microscopy; (180.4315) Nonlinear microscopy

Year: 2013 PMID： 24156043 PMCID： PMC3799645 DOI： 10.1364/BOE.4.001786

Source DB: PubMed Journal: Biomed Opt Express ISSN： 2156-7085 Impact factor: 3.732

Keyword Cloud
References

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Hyper-numerical aperture (NA = 2.8) microscope using λ = 1.56 µm femtosecond source for multi-photon imaging.

Review 1. Two-photon excitation fluorescence microscopy.

2. Illumination artifacts in hyper-NA vector imaging.

3. Third-harmonic generation from single gold nanoparticles.

4. Third-harmonic generation microscopy by use of a compact, femtosecond fiber laser source.

5. Third harmonic generation microscopy.

6. Label-free multi-photon imaging using a compact femtosecond fiber laser mode-locked by carbon nanotube saturable absorber.

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