Literature DB >> 11701518

Two-photon excitation fluorescence microscopy.

P T So1, C Y Dong, B R Masters, K M Berland.   

Abstract

Two-photon fluorescence microscopy is one of the most important recent inventions in biological imaging. This technology enables noninvasive study of biological specimens in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced specimen photodamage and enhanced penetration depth. It also produces higher-contrast images and is a novel method to trigger localized photochemical reactions. Two-photon microscopy continues to find an increasing number of applications in biology and medicine.

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Year:  2000        PMID： 11701518     DOI： 10.1146/annurev.bioeng.2.1.399

Source DB:  PubMed          Journal:  Annu Rev Biomed Eng        ISSN： 1523-9829            Impact factor:   9.590


  174 in total

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