Literature DB >> 2414739

Low ionic strength extraction of nuclease-treated nuclei destroys the attachment of transcriptionally active DNA to the nuclear skeleton.

S V Razin, O V Yarovaya, G P Georgiev.   

Abstract

We have studied how the conditions in which the nuclear matrix is isolated influence the association of transcribing DNA with the nuclear matrix. Extraction of nuclease-treated nuclei with a low ionic strength solution before a high salt nuclei with a low ionic strength solution before a high salt extraction completely abolishes this association. However, RNA removal by RNAase treatment does not affect the binding of transcribing DNA to the nuclear matrix. The nature of the association of active genes with the nuclear matrix is discussed.

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Year:  1985        PMID： 2414739      PMCID： PMC322053          DOI： 10.1093/nar/13.20.7427

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  23 in total

1.  [Localization of regions of DNA attachment to the nuclear skeleton within chicken alpha-globin genes in functionally active and functionally inactive nuclei].

Authors:  S V Razin; J Rzeszowska-Wolny; J Moreau; K Scherrer
Journal:  Mol Biol (Mosk)       Date:  1985 Mar-Apr

2.  The association of transcribed genes with the nuclear matrix of Drosophila cells during heat shock.

Authors:  D Small; B Nelkin; B Vogelstein
Journal:  Nucleic Acids Res       Date:  1985-04-11       Impact factor: 16.971

3.  Isolation and characterization of the nuclear matrix in Friend erythroleukemia cells: chromatin and hnRNA interactions with the nuclear matrix.

Authors:  B H Long; C Y Huang; A O Pogo
Journal:  Cell       Date:  1979-12       Impact factor: 41.582

4.  Protein-depleted chromosomes. II. Experiments concerning the reality of chromosome scaffolds.

Authors:  G Hadlaczky; A T Sumner; A Ross
Journal:  Chromosoma       Date:  1981       Impact factor: 4.316

5.  Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold.

Authors:  J Mirkovitch; M E Mirault; U K Laemmli
Journal:  Cell       Date:  1984-11       Impact factor: 41.582

6.  Initiated complexes of RNA polymerase II are concentrated in the nuclear skeleton associated DNA.

Authors:  S V Razin; O V Yarovaya
Journal:  Exp Cell Res       Date:  1985-05       Impact factor: 3.905

7.  Mapping sequences in loops of nuclear DNA by their progressive detachment from the nuclear cage.

Authors:  P R Cook; I A Brazell
Journal:  Nucleic Acids Res       Date:  1980-07-11       Impact factor: 16.971

8.  Considerations in the isolation of rat liver nuclear matrix, nuclear envelope, and pore complex lamina.

Authors:  S H Kaufmann; D S Coffey; J H Shaper
Journal:  Exp Cell Res       Date:  1981-03       Impact factor: 3.905

9.  Isolation of an active transcription initiation complex from HeLa cell-free extract.

Authors:  H E Tolunay; L Yang; W F Anderson; B Safer
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

10.  hnRNA and its attachment to a nuclear protein matrix.

Authors:  C A van Eekelen; W J van Venrooij
Journal:  J Cell Biol       Date:  1981-03       Impact factor: 10.539
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  13 in total

1.  Gene positional changes relative to the nuclear substructure correlate with the proliferating status of hepatocytes during liver regeneration.

Authors:  Apolinar Maya-Mendoza; Rolando Hernández-Muñoz; Patricio Gariglio; Armando Aranda-Anzaldo
Journal:  Nucleic Acids Res       Date:  2003-11-01       Impact factor: 16.971

2.  Nucleoprotein hybridization: a method for isolating active and inactive genes as chromatin.

Authors:  C Vincenz; J Fronk; G A Tank; J P Langmore
Journal:  Nucleic Acids Res       Date:  1991-03-25       Impact factor: 16.971

3.  Subnuclear localization of the trans-activating protein of human T-cell leukemia virus type I.

Authors:  D J Slamon; W J Boyle; D E Keith; M F Press; D W Golde; L M Souza
Journal:  J Virol       Date:  1988-03       Impact factor: 5.103

4.  Mapping of structural and transcription-related matrix attachment sites in the alpha-globin gene domain of avian erythroblasts and erythrocytes.

Authors:  G Farache; S V Razin; J Rzeszowska-Wolny; J Moreau; F R Targa; K Scherrer
Journal:  Mol Cell Biol       Date:  1990-10       Impact factor: 4.272

5.  Nuclear translocation and carboxyl-terminal domain phosphorylation of RNA polymerase II delineate the two phases of zygotic gene activation in mammalian embryos.

Authors:  S Bellier; S Chastant; P Adenot; M Vincent; J P Renard; O Bensaude
Journal:  EMBO J       Date:  1997-10-15       Impact factor: 11.598

6.  Recruitment of damaged DNA to the nuclear matrix in hamster cells following ultraviolet irradiation.

Authors:  D R Koehler; P C Hanawalt
Journal:  Nucleic Acids Res       Date:  1996-08-01       Impact factor: 16.971

7.  Cytochemical localization of DNA loop attachment sites to the nuclear lamina and to the inner nuclear matrix.

Authors:  N Zini; G Mazzotti; P Santi; R Rizzoli; A Galanzi; R Rana; N M Maraldi
Journal:  Histochemistry       Date:  1989

8.  Replication origins are attached to the nuclear skeleton.

Authors:  S V Razin; M G Kekelidze; E M Lukanidin; K Scherrer; G P Georgiev
Journal:  Nucleic Acids Res       Date:  1986-10-24       Impact factor: 16.971

9.  The distribution of tightly bound proteins along the DNA chain reflects the type of cell differentiation.

Authors:  S V Razin; V V Chernokhvostov; E S Vassetzky; M V Razina; G P Georgiev
Journal:  Nucleic Acids Res       Date:  1988-05-11       Impact factor: 16.971

10.  The structure of nucleosomal core particles within transcribed and repressed gene regions.

Authors:  V M Studitsky; A V Belyavsky; A F Melnikova; A D Mirzabekov
Journal:  Nucleic Acids Res       Date:  1988-12-09       Impact factor: 16.971
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