AIM: Epigenetic modifications, such as DNA methylation, are considered important in the regulation of target genes in cancer development. 5-Hydroxymethylcytosine (5hmC) was recently discovered to be related to the process of malignant transformation. The influence of DNA methylation in oral squamous cell carcinomas (OSCC) is not fully-understood. Therefore, the aim of the present study was to investigate the DNA methylation pattern in OSCC compared to healthy oral epithelium. MATERIALS AND METHODS: Oral mucosal samples from patients with OSCC (n=15) and healthy mucosa (n=12) were analyzed using immunohistochemistry with antibodies against 5hmC, 5mC and ten-eleven-translocation-2 (TET2). RESULTS: A significant decrease in 5hmC and TET2 expression was found in OSCC compared to healthy oral epithelium. In contrast, there was a significant increase in 5mC expression in OSCC compared to healthy epithelium. CONCLUSION: Our results indicate that loss of 5hmC is an epigenetic event of OSCC.
AIM: Epigenetic modifications, such as DNA methylation, are considered important in the regulation of target genes in cancer development. 5-Hydroxymethylcytosine (5hmC) was recently discovered to be related to the process of malignant transformation. The influence of DNA methylation in oral squamous cell carcinomas (OSCC) is not fully-understood. Therefore, the aim of the present study was to investigate the DNA methylation pattern in OSCC compared to healthy oral epithelium. MATERIALS AND METHODS: Oral mucosal samples from patients with OSCC (n=15) and healthy mucosa (n=12) were analyzed using immunohistochemistry with antibodies against 5hmC, 5mC and ten-eleven-translocation-2 (TET2). RESULTS: A significant decrease in 5hmC and TET2 expression was found in OSCC compared to healthy oral epithelium. In contrast, there was a significant increase in 5mC expression in OSCC compared to healthy epithelium. CONCLUSION: Our results indicate that loss of 5hmC is an epigenetic event of OSCC.
Entities:
Keywords:
5-hydoxymethylcytosine; DNA methylation; Oral cancer; biological markers; ten-eleven-translocation 2
Authors: Christopher R Getchell; Eric T McCarthy; Douglass W Tucker; Anders W Ohman; Naoko Sasamoto; Shuyun Xu; Joo Yeon Ko; Mamta Gupta; Amy Shafrir; Jamie E Medina; Jonathan J Lee; Lauren A MacDonald; Ammara Malik; Kathleen T Hasselblatt; Wenjing Li; Hong Zhang; Samuel J Kaplan; George F Murphy; Michelle S Hirsch; Joyce F Liu; Ursula A Matulonis; Kathryn L Terry; Christine G Lian; Daniela M Dinulescu Journal: Clin Cancer Res Date: 2017-12-20 Impact factor: 12.531
Authors: Fengju Song; Christopher I Amos; Jeffrey E Lee; Christine G Lian; Shenying Fang; Hongliang Liu; Stuart MacGregor; Mark M Iles; Matthew H Law; Neal I Lindeman; Grant W Montgomery; David L Duffy; Anne E Cust; Mark A Jenkins; David C Whiteman; Richard F Kefford; Graham G Giles; Bruce K Armstrong; Joanne F Aitken; John L Hopper; Kevin M Brown; Nicholas G Martin; Graham J Mann; D Timothy Bishop; Julia A Newton Bishop; Peter Kraft; Abrar A Qureshi; Peter A Kanetsky; Nicholas K Hayward; David J Hunter; Qingyi Wei; Jiali Han Journal: Carcinogenesis Date: 2014-06-30 Impact factor: 4.944
Authors: Theo F J Kraus; Andrea Greiner; Martina Steinmaurer; Vanessa Dietinger; Virginie Guibourt; Hans A Kretzschmar Journal: J Cancer Date: 2015-07-15 Impact factor: 4.207