Bibi Sedigheh Fazly Bazzaz1, Bahman Khameneh2, Mohammad-mehdi Jalili-Behabadi3, Bizhan Malaekeh-Nikouei4, Seyed Ahmad Mohajeri5. 1. Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. 2. Students Research Committee, Department of Food and Drug Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. 3. Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. 4. Nanotechnology Research Centre, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. 5. Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: mohajeria@mums.ac.ir.
Abstract
PURPOSE: Contact lenses that incorporate antimicrobial properties may reduce the risk for microbial-associated adverse events for lens wearers. The aim of this study was to assess the antimicrobial effects of silver nanoparticles (NP) when impregnated in a hydrogel material. METHODS: Hydrogel disks, used as a proxy for soft contact lenses, were prepared with silver NPs to add an antimicrobial effect to the polymer. Six groups of disks were created, each with a different concentration of silver NPs. The antimicrobial effect of the hydrogels against Pseudomonas aeruginosa (ATCC15442) and Staphylococcus aureus (ATCC6538) was evaluated at 6, 24, 48 and 72 h. RESULTS: Silver NP concentrations ranged from 20.71 to 98.06 μg/disk. All groups demonstrated excellent antibacterial effects against P. aeruginosa at each time point. After 6h all disks didn't exhibit desirable antibacterial activity against S. aureus; whereas except those with 20.71 μg silver NPs showed antibacterial activity at 24h and only the disks with 57.13 and 98.06 μg silver NPs showed antimicrobial activity at 48 and 72 h. CONCLUSIONS: The development of contact lenses made of a silver NP-impregnated hydrogel material may bring antimicrobial effects sufficient to decrease the risk of microbial-related adverse events for lens wearers.
PURPOSE: Contact lenses that incorporate antimicrobial properties may reduce the risk for microbial-associated adverse events for lens wearers. The aim of this study was to assess the antimicrobial effects of silver nanoparticles (NP) when impregnated in a hydrogel material. METHODS: Hydrogel disks, used as a proxy for soft contact lenses, were prepared with silver NPs to add an antimicrobial effect to the polymer. Six groups of disks were created, each with a different concentration of silver NPs. The antimicrobial effect of the hydrogels against Pseudomonas aeruginosa (ATCC15442) and Staphylococcus aureus (ATCC6538) was evaluated at 6, 24, 48 and 72 h. RESULTS:Silver NP concentrations ranged from 20.71 to 98.06 μg/disk. All groups demonstrated excellent antibacterial effects against P. aeruginosa at each time point. After 6h all disks didn't exhibit desirable antibacterial activity against S. aureus; whereas except those with 20.71 μg silver NPs showed antibacterial activity at 24h and only the disks with 57.13 and 98.06 μg silver NPs showed antimicrobial activity at 48 and 72 h. CONCLUSIONS: The development of contact lenses made of a silver NP-impregnated hydrogel material may bring antimicrobial effects sufficient to decrease the risk of microbial-related adverse events for lens wearers.
Authors: Edyta B Hendiger; Marcin Padzik; Ines Sifaoui; María Reyes-Batlle; Atteneri López-Arencibia; Aitor Rizo-Liendo; Carlos J Bethencourt-Estrella; Desirée San Nicolás-Hernández; Olfa Chiboub; Rubén L Rodríguez-Expósito; Marta Grodzik; Anna Pietruczuk-Padzik; Karolina Stępień; Gabriela Olędzka; Lidia Chomicz; José E Piñero; Jacob Lorenzo-Morales Journal: Pathogens Date: 2020-05-05