PROBLEM: The function of IL-10 producing regulatory B cells (Breg) during gestation is unknown. Here, we aimed to understand their participation in early pregnancy. METHOD: CD19(+) CD24(hi) CD27(+) B cell frequency, measured by flow cytometry, increased with pregnancy onset but not in the case of spontaneous abortions. RESULTS: B cells from non-pregnant women cultured with serum from normal pregnant women produced higher IL-10 levels than those cultured with serum from spontaneous abortion patients or autologous serum. CD19(+) -activated B cells from pregnant women strongly suppressed TNF-a production by CD4(+) T cells when cocultured. We identified hCG as an important factor regulating the number and function of Breg during pregnancy. CONCLUSIONS: Breg emerge as important players in pregnancy; they suppress undesired immune responses from maternal T cells and are therefore important for tolerance acquisition.
PROBLEM: The function of IL-10 producing regulatory B cells (Breg) during gestation is unknown. Here, we aimed to understand their participation in early pregnancy. METHOD:CD19(+) CD24(hi) CD27(+) B cell frequency, measured by flow cytometry, increased with pregnancy onset but not in the case of spontaneous abortions. RESULTS: B cells from non-pregnant women cultured with serum from normal pregnant women produced higher IL-10 levels than those cultured with serum from spontaneous abortionpatients or autologous serum. CD19(+) -activated B cells from pregnant women strongly suppressed TNF-a production by CD4(+) T cells when cocultured. We identified hCG as an important factor regulating the number and function of Breg during pregnancy. CONCLUSIONS: Breg emerge as important players in pregnancy; they suppress undesired immune responses from maternal T cells and are therefore important for tolerance acquisition.
Authors: Ruth Marian Guzman-Genuino; Tanya Dimova; Yuan You; Paulomi Aldo; John D Hayball; Gil Mor; Kerrilyn R Diener Journal: Am J Reprod Immunol Date: 2019-10-06 Impact factor: 3.886
Authors: Diego Catalán; Miguel Andrés Mansilla; Ashley Ferrier; Lilian Soto; Kristine Oleinika; Juan Carlos Aguillón; Octavio Aravena Journal: Front Immunol Date: 2021-04-29 Impact factor: 7.561
Authors: Lynsa M Nguyen; Joel I Omage; Kristen Noble; Kelsey L McNew; Daniel J Moore; David M Aronoff; Ryan S Doster Journal: Am J Reprod Immunol Date: 2021-10-19 Impact factor: 3.886