Martin Niklas1, Amir Abdollahi, Mark S Akselrod, Jürgen Debus, Oliver Jäkel, Steffen Greilich. 1. Division of Medical Physics in Radiation Oncology, German Cancer Research Center, Heidelberg, Germany; German Cancer Consortium, National Center for Radiation Research in Oncology, Heidelberg Institute of Radiation Oncology, Heidelberg, Germany. Electronic address: m.niklas@dkfz.de.
Abstract
PURPOSE: To report on the spatial correlation of physical track information (fluorescent nuclear track detectors, FNTDs) and cellular DNA damage response by using a novel hybrid detector (Cell-Fit-HD). METHODS AND MATERIALS: The FNTDs were coated with a monolayer of human non-small cell lung carcinoma (A549) cells and irradiated with carbon ions (270.55 MeV u(-1), rising flank of the Bragg peak). Phosphorylated histone variant H2AX accumulating at the irradiation-induced double-strand break site was labeled (RIF). The position and direction of ion tracks in the FNTD were registered with the location of the RIF sequence as an ion track surrogate in the cell layer. RESULTS: All RIF sequences could be related to their corresponding ion tracks, with mean deviations of 1.09 μm and -1.72 μm in position and of 2.38° in slope. The mean perpendicular between ion track and RIF sequence was 1.58 μm. The mean spacing of neighboring RIFs exhibited a regular rather than random spacing. CONCLUSIONS: Cell-Fit-HD allows for unambiguous spatial correlation studies of cell damage with respect to the intracellular ion traversal under therapeutic beam conditions.
PURPOSE: To report on the spatial correlation of physical track information (fluorescent nuclear track detectors, FNTDs) and cellular DNA damage response by using a novel hybrid detector (Cell-Fit-HD). METHODS AND MATERIALS: The FNTDs were coated with a monolayer of human non-small cell lung carcinoma (A549) cells and irradiated with carbon ions (270.55 MeV u(-1), rising flank of the Bragg peak). Phosphorylated histone variant H2AX accumulating at the irradiation-induced double-strand break site was labeled (RIF). The position and direction of ion tracks in the FNTD were registered with the location of the RIF sequence as an ion track surrogate in the cell layer. RESULTS: All RIF sequences could be related to their corresponding ion tracks, with mean deviations of 1.09 μm and -1.72 μm in position and of 2.38° in slope. The mean perpendicular between ion track and RIF sequence was 1.58 μm. The mean spacing of neighboring RIFs exhibited a regular rather than random spacing. CONCLUSIONS: Cell-Fit-HD allows for unambiguous spatial correlation studies of cell damage with respect to the intracellular ion traversal under therapeutic beam conditions.
Authors: Gabriel O Sawakuchi; Felisberto A Ferreira; Conor H McFadden; Timothy M Hallacy; Dal A Granville; Narayan Sahoo; Mark S Akselrod Journal: Med Phys Date: 2016-05 Impact factor: 4.071
Authors: Martin Niklas; Julian Schlegel; Hans Liew; Ferdinand Zimmermann; Katrin Rein; Dietrich W M Walsh; Oleh Dzyubachyk; Tim Holland-Letz; Shirin Rahmanian; Steffen Greilich; Armin Runz; Oliver Jäkel; Jürgen Debus; Amir Abdollahi Journal: Cell Rep Methods Date: 2022-02-17
Authors: Ivana Dokic; Martin Niklas; Ferdinand Zimmermann; Andrea Mairani; Philipp Seidel; Damir Krunic; Oliver Jäkel; Jürgen Debus; Steffen Greilich; Amir Abdollahi Journal: Front Oncol Date: 2015-12-07 Impact factor: 6.244
Authors: Ivana Dokic; Andrea Mairani; Martin Niklas; Ferdinand Zimmermann; Naved Chaudhri; Damir Krunic; Thomas Tessonnier; Alfredo Ferrari; Katia Parodi; Oliver Jäkel; Jürgen Debus; Thomas Haberer; Amir Abdollahi Journal: Oncotarget Date: 2016-08-30