Literature DB >> 2410265

Rapid detection of Vibrio cholerae O:1 by motility inhibition and immunofluorescence with monoclonal antibodies.

B Gustafsson, T Holme.   

Abstract

Monoclonal antibodies against the group and type specific antigens of Vibrio cholerae O:1 lipopolysaccharide were used for the rapid detection of Vibrio cholerae strains by motility inhibition and immunofluorescence. Motility inhibition of live Vibrio cholerae O:1 was obtained with group specific monoclonal antibodies. Monoclonal antibodies against the type specific antigens B (Ogawa) and C (Inaba) inhibited motility of strains of homologous serotypes only. Indirect immunofluorescence of heatfixed bacteria with monoclonal antibodies and fluorescein-isothiocyanate conjugated rabbit anti-mouse immunoglobulin was also shown to be suitable for the rapid detection of Vibrio cholerae O:1. Both tests were highly specific and no cross-reactions were observed with strains of non-O:1 vibrios, Escherichia coli or Salmonella spp. tested. However, a weak fluorescence of some Ogawa strains was observed when high concentrations of Inaba specific monoclonal antibodies were used.

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Year:  1985        PMID： 2410265     DOI： 10.1007/bf02013655

Source DB:  PubMed          Journal:  Eur J Clin Microbiol        ISSN： 0722-2211            Impact factor:   3.267


  11 in total

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5.  Growth of primary plasmacytomas in the mineral oil-conditioned peritoneal environment.

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Authors:  B Gustafsson; T Holme
Journal:  J Clin Microbiol       Date:  1983-09       Impact factor: 5.948

8.  Monoclonal antibodies against Vibrio cholerae lipopolysaccharide.

Authors:  B Gustafsson; A Rosén; T Holme
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9.  RAPID IDENTIFICATION OF VIBRIO CHOLERAE BY DARKFIELD MICROSCOPY.

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Authors:  B Gustafsson
Journal:  J Clin Microbiol       Date:  1984-12       Impact factor: 5.948
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Review 4.  Cholera.

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9.  Passive Immunity to Vibrio cholerae O1 Afforded by a Human Monoclonal IgA1 Antibody Expressed in Milk.

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  9 in total

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