Emiko Noguchi1, Rina Kato2, Kayoko Ohno3, Atsushi Mitsui2, Takashi Obama2, Tsutomu Hirano3, Hiroyuki Itabe4, Matsuo Yamamoto5. 1. Department of Periodontology, Showa University School of Dentistry, Kitasenzoku, Ota-ku, Tokyo 145-8515, Japan; Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. 2. Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. 3. Division of Diabetes, Metabolism and Endocrinology, Department of Medicine, Showa University School of Medicine, Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. 4. Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. Electronic address: h-itabe@pharm.showa-u.ac.jp. 5. Department of Periodontology, Showa University School of Dentistry, Kitasenzoku, Ota-ku, Tokyo 145-8515, Japan.
Abstract
OBJECTIVE: Oral health conditions have a significant relationship with diabetes mellitus (DM) as well as dyslipidemia. In this study, we investigated the levels of apolipoprotein B (apoB) and oxidized low-density lipoprotein (oxLDL) in the gingival crevicular fluid (GCF) from patients with DM. METHODS: GCF and blood samples from 18 DM patients and 18 healthy subjects were examined. GCF was collected with paper points without inflicting any harm. The apoB and oxLDL levels were measured by sandwich ELISA assays. RESULTS: The number of teeth with a deep probing pocket depth and the number of teeth with bleeding on probing, two typical periodontal parameters, correlated with the DM parameters, such as hemoglobin A1c. The GCF volume and the concentrations of protein, apoB and oxLDL in GCF were significantly higher in the DM patients than in the healthy subjects. In particular, the apoB concentration in GCF was increased 6-fold in the DM patients. The GCF apoB concentration correlated well with the DM parameters in plasma. CONCLUSION: GCF could be a clinical source for examining not only the oral status of patients, but also certain systemic conditions.
OBJECTIVE: Oral health conditions have a significant relationship with diabetes mellitus (DM) as well as dyslipidemia. In this study, we investigated the levels of apolipoprotein B (apoB) and oxidized low-density lipoprotein (oxLDL) in the gingival crevicular fluid (GCF) from patients with DM. METHODS: GCF and blood samples from 18 DMpatients and 18 healthy subjects were examined. GCF was collected with paper points without inflicting any harm. The apoB and oxLDL levels were measured by sandwich ELISA assays. RESULTS: The number of teeth with a deep probing pocket depth and the number of teeth with bleeding on probing, two typical periodontal parameters, correlated with the DM parameters, such as hemoglobin A1c. The GCF volume and the concentrations of protein, apoB and oxLDL in GCF were significantly higher in the DMpatients than in the healthy subjects. In particular, the apoB concentration in GCF was increased 6-fold in the DMpatients. The GCF apoB concentration correlated well with the DM parameters in plasma. CONCLUSION: GCF could be a clinical source for examining not only the oral status of patients, but also certain systemic conditions.