Maike Keck1, Johanna Kober2, Otto Riedl2, Hugo B Kitzinger2, Sonja Wolf2, Thomas M Stulnig3, Maximilian Zeyda3, Alfred Gugerell2. 1. Division of Plastic and Reconstructive Surgery, Department of Surgery, and Clinical Division of Endocrinology and Metabolism, Department of Medicine III, Medical University Vienna, Währingergürtel 18-22, 1090 Vienna, Austria. Electronic address: maike.keck@meduniwien.ac.at. 2. Division of Plastic and Reconstructive Surgery, Department of Surgery, and Clinical Division of Endocrinology and Metabolism, Department of Medicine III, Medical University Vienna, Währingergürtel 18-22, 1090 Vienna, Austria. 3. Christian Doppler-Laboratory for Cardio-Metabolic Immunotherapy, Clinical Division of Endocrinology and Metabolism, Department of Medicine III, Medical University Vienna, Vienna, Austria.
Abstract
BACKGROUND: Adipose-derived stem cells (ASCs) play a key role in tissue engineering approaches and are probably of major importance in the context of autologous fat transfer. A number of different tools for harvesting ASCs-containing fat tissue have been established. Such devices should be easy to handle, time saving, low priced, safe and provide a high amount of viable ASCs in the aspirate. Power-assisted liposuction (PAL) has not yet been described in the literature as a tool for fat harvesting for lipotransfer. Aim of this study was to investigate ASCs' viability in fat tissue harvested using PAL versus manual aspiration (MA). METHODS: Fat tissue was obtained from 9 donors undergoing abdominoplasty. Samples were divided into two sections. Out of each section fat was harvested using either PAL or MA. Number of isolated ASCs was defined, proliferation rate was determined and cell viability was assessed by flow cytometry. The ability of isolated ASCs to differentiate into mature adipocytes was analyzed by gene marker expression. RESULTS: The number of viable ASCs and the proliferation rates did not significantly differ between PAL and MA but cells harvested using PAL showed significantly higher expression levels of differentiation markers adiponectin, GLUT4 and PPARg. CONCLUSION: Our results show that PAL is a feasible method for harvesting fat tissue containing viable ASCs. Quantity and quality of PAL-harvested ASC is similar or even better, respectively, compared to ASCs harvested by MA.
BACKGROUND: Adipose-derived stem cells (ASCs) play a key role in tissue engineering approaches and are probably of major importance in the context of autologous fat transfer. A number of different tools for harvesting ASCs-containing fat tissue have been established. Such devices should be easy to handle, time saving, low priced, safe and provide a high amount of viable ASCs in the aspirate. Power-assisted liposuction (PAL) has not yet been described in the literature as a tool for fat harvesting for lipotransfer. Aim of this study was to investigate ASCs' viability in fat tissue harvested using PAL versus manual aspiration (MA). METHODS: Fat tissue was obtained from 9 donors undergoing abdominoplasty. Samples were divided into two sections. Out of each section fat was harvested using either PAL or MA. Number of isolated ASCs was defined, proliferation rate was determined and cell viability was assessed by flow cytometry. The ability of isolated ASCs to differentiate into mature adipocytes was analyzed by gene marker expression. RESULTS: The number of viable ASCs and the proliferation rates did not significantly differ between PAL and MA but cells harvested using PAL showed significantly higher expression levels of differentiation markers adiponectin, GLUT4 and PPARg. CONCLUSION: Our results show that PAL is a feasible method for harvesting fat tissue containing viable ASCs. Quantity and quality of PAL-harvested ASC is similar or even better, respectively, compared to ASCs harvested by MA.
Authors: Jeffrey T Krawiec; Han-Tsung Liao; LaiYee Lily Kwan; Antonio D'Amore; Justin S Weinbaum; J Peter Rubin; William R Wagner; David A Vorp Journal: J Vasc Surg Date: 2016-12-22 Impact factor: 4.268
Authors: Dominik Duscher; Anna Luan; Robert C Rennert; David Atashroo; Zeshaan N Maan; Elizabeth A Brett; Alexander J Whittam; Natalie Ho; Michelle Lin; Michael S Hu; Graham G Walmsley; Raphael Wenny; Manfred Schmidt; Arndt F Schilling; Hans-Günther Machens; Georg M Huemer; Derrick C Wan; Michael T Longaker; Geoffrey C Gurtner Journal: J Transl Med Date: 2016-05-06 Impact factor: 5.531
Authors: Dominik Duscher; David Atashroo; Zeshaan N Maan; Anna Luan; Elizabeth A Brett; Janos Barrera; Sacha M Khong; Elizabeth R Zielins; Alexander J Whittam; Michael S Hu; Graham G Walmsley; Michael S Pollhammer; Manfred Schmidt; Arndt F Schilling; Hans-Günther Machens; Georg M Huemer; Derrick C Wan; Michael T Longaker; Geoffrey C Gurtner Journal: Stem Cells Transl Med Date: 2015-12-23 Impact factor: 6.940