Literature DB >> 2409172

Triple immunological staining with colloidal gold, fluorescein and rhodamine as labels.

J J Van Dongen, H Hooijkaas, W M Comans-Bitter, K Benne, T M Van Os, J De Josselin de Jong.   

Abstract

Colloidal gold particles are detectable by light microscopy with polarized light in the same epi-illumination system as for fluorescence microscopy. Colloidal gold particles can thus be used in combination with a fluorochrome for the combined immunological detection of surface membrane and cytoplasmic markers. We analyzed human bone marrow cells by a triple immunological staining for 3 different markers, using colloidal gold, fluorescein and rhodamine as labels. Our results demonstrate that such a triple immunological staining provides a powerful tool for study of the heterogeneity of small cell populations.

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Year:  1985        PMID： 2409172     DOI： 10.1016/0022-1759(85)90157-7

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  3 in total

Review 1.  Immunogold labelling of neuroendocrine peptides with special reference to antibody specificity and multiple staining techniques.

Authors:  L I Larsson
Journal:  Histochem Cell Biol       Date:  1996-07       Impact factor: 4.304

2.  Determination of rosuvastatin in urine by spectrofluorimetry after liquid-liquid extraction and derivatization in acidic medium.

Authors:  Victor S M Braga; Taiza P Mancilha; Ricardo J Cassella; Wagner F Pacheco
Journal:  J Fluoresc       Date:  2012-08-18       Impact factor: 2.217

Review 3.  Phenotypic and genotypic characteristics of the human prothymocyte.

Authors:  J J van Dongen; W M Comans-Bitter
Journal:  Immunol Res       Date:  1987       Impact factor: 2.829
  3 in total

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