Literature DB >> 24075879

Selection of Pseudomonas aeruginosa reference genes for RT-qPCR analysis from sputum of cystic fibrosis patients.

Patricia Costaglioli1, Christophe Barthe, Michael Fayon, Naik Christoflour, Stéphanie Bui, Laurence Derlich, Philippe Domblides, Marc Crouzet, Sébastien Vilain, Bertrand Garbay.   

Abstract

The prerequisite to monitor gene expression is the selection of reference genes for normalization of RT-qPCR results. Using 13 sputum samples collected from 9 CF patients, we demonstrated that PA2875 and PA3340 are better reference genes than the previously used clpX and oprL genes.
Copyright © 2013 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  Cystic fibrosis; Pseudomonas aeruginosa; RT-qPCR; Reference genes; Sputum

Mesh：

Year:  2013        PMID： 24075879     DOI： 10.1016/j.mcp.2013.09.003

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN： 0890-8508            Impact factor:   2.365


  3 in total

1.  Availability of Zinc Impacts Interactions between Streptococcus sanguinis and Pseudomonas aeruginosa in Coculture.

Authors:  Kewei Li; Alex H Gifford; Thomas H Hampton; George A O'Toole
Journal:  J Bacteriol       Date:  2020-01-02       Impact factor: 3.490

2.  Rapid detection of four non-fermenting Gram-negative bacteria directly from cystic fibrosis patient's respiratory samples on the BD MAX™ system.

Authors:  Talita T Rocchetti; Suzane Silbert; Alicia Gostnell; Carly Kubasek; Robert Jerris; Jennifer Vong; Raymond Widen
Journal:  Pract Lab Med       Date:  2018-05-24

3.  Selection and Evaluation of Reference Genes for Reverse Transcription-Quantitative PCR Expression Studies in a Thermophilic Bacterium Grown under Different Culture Conditions.

Authors:  Kathleen D Cusick; Lisa A Fitzgerald; Allison L Cockrell; Justin C Biffinger
Journal:  PLoS One       Date:  2015-06-26       Impact factor: 3.240
  3 in total

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