Literature DB >> 2407234

The Escherichia coli cysG gene encodes S-adenosylmethionine-dependent uroporphyrinogen III methylase.

M J Warren1, C A Roessner, P J Santander, A I Scott.   

Abstract

The Escherichia coli cysG gene was successfully subcloned and over-expressed to produce a 52 kDa protein that was purified to homogeneity. This protein was shown to catalyse the S-adenosylmethionine-dependent methylation of uroporphyrinogen III to give a product identified as sirohydrochlorin on the basis of its absorption spectra, incorporation of 14C label from S-adenosyl[Me-14C]methionine and mass and 1H-n.m.r. spectra of its octamethyl ester. Further confirmation of the structure was obtained from a 14C-n.m.r. spectrum of the methyl ester produced by incubation of the methylase with uroporphyrinogen III, derived from [4.6-13C2]porphobilinogen, and S-adenosyl[Me-13C]methionine.

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Year:  1990        PMID： 2407234      PMCID： PMC1133693          DOI： 10.1042/bj2650725

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  8 in total

1.  Molecular cloning and functional analysis of the cysG and nirB genes of Escherichia coli K12, two closely-linked genes required for NADH-dependent nitrite reductase activity.

Authors:  H Macdonald; J Cole
Journal:  Mol Gen Genet       Date:  1985

2.  Site-directed mutagenesis and high-resolution NMR spectroscopy of the active site of porphobilinogen deaminase.

Authors:  A I Scott; C A Roessner; N J Stolowich; P Karuso; H J Williams; S K Grant; M D Gonzalez; T Hoshino
Journal:  Biochemistry       Date:  1988-10-18       Impact factor: 3.162

3.  Mechanism of action of porphobilinogen deaminase. The participation of stable enzyme substrate covalent intermediates between porphobilinogen and the porphobilinogen deaminase from Rhodopseudomonas spheroides.

Authors:  P M Jordan; A Berry
Journal:  Biochem J       Date:  1981-04-01       Impact factor: 3.857

4.  Maturation of the head of bacteriophage T4. I. DNA packaging events.

Authors:  U K Laemmli; M Favre
Journal:  J Mol Biol       Date:  1973-11-15       Impact factor: 5.469

5.  Reduced nicotinamide adenine dinucleotide phosphate-sulfite reductase of enterobacteria. II. Identification of a new class of heme prosthetic group: an iron-tetrahydroporphyrin (isobacteriochlorin type) with eight carboxylic acid groups.

Authors:  M J Murphy; L M Siegel; H Kamin; D Rosenthal
Journal:  J Biol Chem       Date:  1973-04-25       Impact factor: 5.157

6.  Salmonella typhimurium synthesizes cobalamin (vitamin B12) de novo under anaerobic growth conditions.

Authors:  R M Jeter; B M Olivera; J R Roth
Journal:  J Bacteriol       Date:  1984-07       Impact factor: 3.490

7.  Biochemical and genetic characterization of nirB mutants of Escherichia coli K 12 pleiotropically defective in nitrite and sulphite reduction.

Authors:  J A Cole; B M Newman; P White
Journal:  J Gen Microbiol       Date:  1980-10

8.  [On cobyrinic acid biosynthesis. Novel methylated hydroporphyrins and their role in cobyrinis acid formation (author's transl)].

Authors:  R Deeg; H P Kriemler; K H Bergmann; G Müller
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1977-03
  8 in total
  33 in total

1.  The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase.

Authors:  Heidi L Schubert; Evelyne Raux; Amanda A Brindley; Helen K Leech; Keith S Wilson; Christopher P Hill; Martin J Warren
Journal:  EMBO J       Date:  2002-05-01       Impact factor: 11.598

2.  Crystal structure of the heme d1 biosynthesis enzyme NirE in complex with its substrate reveals new insights into the catalytic mechanism of S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferases.

Authors:  Sonja Storbeck; Sayantan Saha; Joern Krausze; Björn U Klink; Dirk W Heinz; Gunhild Layer
Journal:  J Biol Chem       Date:  2011-05-31       Impact factor: 5.157

3.  Effect of mutations in the transmethylase and dehydrogenase/chelatase domains of sirohaem synthase (CysG) on sirohaem and cobalamin biosynthesis.

Authors:  S C Woodcock; E Raux; F Levillayer; C Thermes; A Rambach; M J Warren
Journal:  Biochem J       Date:  1998-02-15       Impact factor: 3.857

Review 4.  Siroheme: an essential component for life on earth.

Authors:  Baishnab C Tripathy; Irena Sherameti; Ralf Oelmüller
Journal:  Plant Signal Behav       Date:  2010-01

5.  Anaerobic protoporphyrin biosynthesis does not require incorporation of methyl groups from methionine.

Authors:  D W Bollivar; T Elliott; S I Beale
Journal:  J Bacteriol       Date:  1995-10       Impact factor: 3.490

6.  Structure and function of SirC from Bacillus megaterium: a metal-binding precorrin-2 dehydrogenase.

Authors:  Heidi L Schubert; Ruth S Rose; Helen K Leech; Amanda A Brindley; Christopher P Hill; Stephen E J Rigby; Martin J Warren
Journal:  Biochem J       Date:  2008-10-15       Impact factor: 3.857

7.  One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies.

Authors:  Yong Chang; David Mead; Vinay Dhodda; Phil Brumm; Brian G Fox
Journal:  Protein Sci       Date:  2009-11       Impact factor: 6.725

8.  Primary structure, expression in Escherichia coli, and properties of S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase from Bacillus megaterium.

Authors:  C Robin; F Blanche; L Cauchois; B Cameron; M Couder; J Crouzet
Journal:  J Bacteriol       Date:  1991-08       Impact factor: 3.490

Review 9.  Functions of the gene products of Escherichia coli.

Authors:  M Riley
Journal:  Microbiol Rev       Date:  1993-12

10.  Two divergent MET10 genes, one from Saccharomyces cerevisiae and one from Saccharomyces carlsbergensis, encode the alpha subunit of sulfite reductase and specify potential binding sites for FAD and NADPH.

Authors:  J Hansen; H Cherest; M C Kielland-Brandt
Journal:  J Bacteriol       Date:  1994-10       Impact factor: 3.490
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