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Literature DB >> 24071916

A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude.

Abhinav P Acharya¹, Parsa M Nafisi, Austin Gardner, Joanna L Mackay, Kousik Kundu, Sanjay Kumar, Niren Murthy.

Abstract

The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.

Entities: Chemical Disease Gene Species

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Year: 2013 PMID： 24071916 PMCID： PMC4011665 DOI： 10.1039/c3cc44783a

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

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6. Mechanism of reaction of hydrogen peroxide with horseradish peroxidase: identification of intermediates in the catalytic cycle.

Authors: J N Rodríguez-López; D J Lowe; J Hernández-Ruiz; A N Hiner; F García-Cánovas; R N Thorneley
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7. Development and application of a near-infrared fluorescence probe for oxidative stress based on differential reactivity of linked cyanine dyes.

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A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude.

1. Development of a near-infrared fluorescence ELISA method using tyramide signal amplification.

2. A genetic strategy for the dynamic and graded control of cell mechanics, motility, and matrix remodeling.

3. Detection of protein analytes via nanoparticle-based bio bar code technology.

Review 4. Trends in biomarker research for cancer detection.

5. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.

6. Mechanism of reaction of hydrogen peroxide with horseradish peroxidase: identification of intermediates in the catalytic cycle.

7. Development and application of a near-infrared fluorescence probe for oxidative stress based on differential reactivity of linked cyanine dyes.

8. Nanoparticle-based bio-bar codes for the ultrasensitive detection of proteins.

Review 9. Horseradish peroxidase: a valuable tool in biotechnology.

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