| Literature DB >> 22490466 |
Haibiao Gong1, Mark Cradduck, Lael Cheung, D Michael Olive.
Abstract
In this study, we applied tyramide signal amplification (TSA) to fluorescence enzyme-linked immunosorbent assay (ELISA) employing horseradish peroxidase (HRP) as the detection enzyme. When used with a human epidermal growth factor ELISA kit, the TSA method led to a >100-fold increase in fluorescence signal intensity in comparison to an unamplified method. It also showed wider dynamic range and better sensitivity compared to a conventional method using tetramethylbenzidine as the HRP substrate.Entities:
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Year: 2012 PMID： 22490466 DOI： 10.1016/j.ab.2012.04.004
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365