Literature DB >> 24056749

A fluorogenic probe for β-galactosidase activity imaging in living cells.

Junyan Han1, Myung Shin Han, Ching-Hsuan Tung.   

Abstract

A cell permeable fluorescence turn-on probe, AcGQCy7, was developed to image β-galactosidase activity in living cells. Once internalized by β-galactosidase-expressing cells, the probe was hydrolyzed into a highly fluorescent molecule, and the fluorescent signal was retained in mitochondria for several days. This resulted in a long-lasting and strong β-galactosidase-dependent intracellular fluorescent signal with little background fluorescence in the culture media.

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Year:  2013        PMID： 24056749      PMCID： PMC3836597          DOI： 10.1039/c3mb70269c

Source DB:  PubMed          Journal:  Mol Biosyst        ISSN： 1742-2051


  34 in total

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