BACKGROUND: Enteroviruses (EVs) are common human viral pathogens, causing a variety of diseases, including aseptic meningitis. Recently, EV aseptic meningitis outbreaks have been reported across Europe, but, in Italy, knowledge of recent EV molecular epidemiology is very limited. OBJECTIVES: We report an outbreak of EV aseptic meningitis in 10 adults in North-Western Italy, from October to November 2012. Patients were parents or close relatives of children <5 years old attending the same class of a nursery school, suffering from a mild febrile upper respiratory disease. Phylogenetic relationship with other European circulating strains was analyzed updating E30 circulation in Italy in recent years. STUDY DESIGN: EVs were detected from cerebrospinal fluid (CSF) specimens with a real-time reverse transcription polymerase chain reaction and virus isolation was achieved from rectal and pharyngeal swabs. For cluster definition and phylogenetic studies, viral VP1 region was directly amplified and sequenced from CSF. RESULTS: EVs were identified in CSF from all patients and from rectal and pharyngeal swabs in 7 of them. Direct sequencing of CSF revealed the presence of the same Echovirus 30 (E30) in all patients and phylogenetic analysis identified it as a diverging clade within E30 genotype VII, the most recent strain circulating in UK, Finland and Denmark since 2006. CONCLUSION: Molecular techniques allowed the rapid identification and typing of E30 from CSF. Phylogenetic analysis revealed that the cluster might be due to a new E30 variant within the genotype VII currently circulating in Europe, thus updating the epidemiology of EV circulation in Italy.
BACKGROUND: Enteroviruses (EVs) are common human viral pathogens, causing a variety of diseases, including aseptic meningitis. Recently, EV aseptic meningitis outbreaks have been reported across Europe, but, in Italy, knowledge of recent EV molecular epidemiology is very limited. OBJECTIVES: We report an outbreak of EV aseptic meningitis in 10 adults in North-Western Italy, from October to November 2012. Patients were parents or close relatives of children <5 years old attending the same class of a nursery school, suffering from a mild febrile upper respiratory disease. Phylogenetic relationship with other European circulating strains was analyzed updating E30 circulation in Italy in recent years. STUDY DESIGN: EVs were detected from cerebrospinal fluid (CSF) specimens with a real-time reverse transcription polymerase chain reaction and virus isolation was achieved from rectal and pharyngeal swabs. For cluster definition and phylogenetic studies, viral VP1 region was directly amplified and sequenced from CSF. RESULTS: EVs were identified in CSF from all patients and from rectal and pharyngeal swabs in 7 of them. Direct sequencing of CSF revealed the presence of the same Echovirus 30 (E30) in all patients and phylogenetic analysis identified it as a diverging clade within E30 genotype VII, the most recent strain circulating in UK, Finland and Denmark since 2006. CONCLUSION: Molecular techniques allowed the rapid identification and typing of E30 from CSF. Phylogenetic analysis revealed that the cluster might be due to a new E30 variant within the genotype VII currently circulating in Europe, thus updating the epidemiology of EV circulation in Italy.
Authors: H Rudolph; R Prieto Dernbach; M Walka; P Rey-Hinterkopf; V Melichar; E Muschiol; S Schweitzer-Krantz; J W Richter; C Weiss; S Böttcher; S Diedrich; H Schroten; T Tenenbaum Journal: Eur J Clin Microbiol Infect Dis Date: 2017-04-13 Impact factor: 3.267
Authors: A Battistone; G Buttinelli; S Fiore; C Amato; P Bonomo; A M Patti; A Vulcano; M Barbi; S Binda; L Pellegrinelli; M L Tanzi; P Affanni; P Castiglia; C Germinario; P Mercurio; A Cicala; M Triassi; F Pennino; L Fiore Journal: Appl Environ Microbiol Date: 2014-08 Impact factor: 4.792
Authors: Kimberley S M Benschop; Eeva K Broberg; Emma Hodcroft; Dennis Schmitz; Jan Albert; Anda Baicus; Jean-Luc Bailly; Gudrun Baldvinsdottir; Natasa Berginc; Soile Blomqvist; Sindy Böttcher; Mia Brytting; Erika Bujaki; Maria Cabrerizo; Cristina Celma; Ondrej Cinek; Eric C J Claas; Jeroen Cremer; Jonathan Dean; Jennifer L Dembinski; Iryna Demchyshyna; Sabine Diedrich; Susanne Dudman; Jake Dunning; Robert Dyrdak; Mary Emmanouil; Agnes Farkas; Cillian De Gascun; Guillaume Fournier; Irina Georgieva; Ruben Gonzalez-Sanz; Jolanda van Hooydonk-Elving; Anne J Jääskeläinen; Ruta Jancauskaite; Kathrin Keeren; Thea K Fischer; Sidsel Krokstad; Lubomira Nikolaeva-Glomb; Ludmila Novakova; Sofie E Midgley; Audrey Mirand; Richard Molenkamp; Ursula Morley; Joël Mossong; Svajune Muralyte; Jean-Luc Murk; Trung Nguyen; Svein A Nordbø; Riikka Österback; Suzan Pas; Laura Pellegrinelli; Vassiliki Pogka; Birgit Prochazka; Petra Rainetova; Marc Van Ranst; Lieuwe Roorda; Isabelle Schuffenecker; Rob Schuurman; Asya Stoyanova; Kate Templeton; Jaco J Verweij; Androniki Voulgari-Kokota; Tytti Vuorinen; Elke Wollants; Katja C Wolthers; Katherina Zakikhany; Richard Neher; Heli Harvala; Peter Simmonds Journal: Emerg Infect Dis Date: 2021-06 Impact factor: 6.883
Authors: Kimberley S M Benschop; Janette C Rahamat-Langendoen; Harrie G A M van der Avoort; Eric C J Claas; Suzan D Pas; Rob Schuurman; Jaco J Verweij; Katja C Wolthers; Hubert G M Niesters; Marion P G Koopmans Journal: Euro Surveill Date: 2016-09-29