Literature DB >> 24038732

Rapid high-specificity microRNA detection using a two-stage isotachophoresis assay.

Giancarlo Garcia-Schwarz1, Juan G Santiago.   

Abstract

Focusing in on the small: A two-stage microRNA detection assay uses electrokinetic focusing to speed up hybridization and a functionalized hydrogel for affinity purification. This method detects microRNAs in 15 minutes with single-nucleotide specificity, and processes only 5 ng of total RNA.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords:  RNA recognition; RNA structures; analytical methods; hybridization assay; isotachophoresis

Mesh：

Substances：

Year:  2013        PMID： 24038732     DOI： 10.1002/anie.201305875

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  8 in total

1.  Sensitive and multiplexed on-chip microRNA profiling in oil-isolated hydrogel chambers.

Authors:  Hyewon Lee; Rathi L Srinivas; Ankur Gupta; Patrick S Doyle
Journal:  Angew Chem Int Ed Engl       Date:  2015-01-07       Impact factor: 15.336

2.  Kinetic fingerprinting to identify and count single nucleic acids.

Authors:  Alexander Johnson-Buck; Xin Su; Maria D Giraldez; Meiping Zhao; Muneesh Tewari; Nils G Walter
Journal:  Nat Biotechnol       Date:  2015-06-22       Impact factor: 54.908

Review 3.  Isotachophoresis: Theory and Microfluidic Applications.

Authors:  Ashwin Ramachandran; Juan G Santiago
Journal:  Chem Rev       Date:  2022-06-22       Impact factor: 72.087

4.  Lateral Flow Test for Visual Detection of Multiple MicroRNAs.

Authors:  Wanli Zheng; Li Yao; Jun Teng; Chao Yan; Panzhu Qin; Guodong Liu; Wei Chen
Journal:  Sens Actuators B Chem       Date:  2018-03-20       Impact factor: 7.460

5.  Low-voltage paper isotachophoresis device for DNA focusing.

Authors:  Xiang Li; Long Luo; Richard M Crooks
Journal:  Lab Chip       Date:  2015-09-04       Impact factor: 6.799

6.  Magnetic ligation method for quantitative detection of microRNAs.

Authors:  Monty Liong; Hyungsoon Im; Maulik D Majmudar; Aaron D Aguirre; Matthew Sebas; Hakho Lee; Ralph Weissleder
Journal:  Adv Healthc Mater       Date:  2014-02-14       Impact factor: 9.933

7.  RNA secondary structured logic gates for profiling the microRNA cancer biomarkers.

Authors:  Mahsa Yazdani; Zohre Beiki; Ali Jahanian
Journal:  IET Nanobiotechnol       Date:  2020-05       Impact factor: 1.847

8.  Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device.

Authors:  Hyewon Lee; Jiseok Lee; Seung-Goo Lee; Patrick S Doyle
Journal:  Anal Chem       Date:  2020-04-02       Impact factor: 6.986
  8 in total

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