OBJECTIVES: Earlier studies involving a priori gene selection have identified promoter regions deregulated by DNA methylation changes in oral squamous cell cancers (OSCCs) and precancers. Interrogation of global DNA methylation patterns for such specimens has not been reported, though such analyses are needed to uncover novel molecular factors driving disease. MATERIALS AND METHODS: We evaluated global DNA methylation patterns for 30 biopsies obtained from 10 patients undergoing surgical removal of an OSCC or carcinoma in situ (CIS). From a disease field in each patient, we collected (i) dysplastic, (ii) CIS or OSCC, and (iii) adjacent normal biopsies. DNA isolated from each biopsy was profiled for methylation status using the Illumina HumanMethylation27K platform. RESULTS: Our data demonstrate that aberrant methylation of promoter CpG islands exists across oral precancer and OSCC genomes. Non-hierarchical clustering of all methylation data revealed distinct methylation patterns between the normal and the CIS/OSCC tissues (with results for dysplastic biopsies split between groups). Multiple genes exhibiting recurrent aberrant DNA methylation were found for both dysplastic and CIS/OSCC groups, and included enrichment for genes found in the WNT and MAPK signaling pathways. CONCLUSION: In identifying aberrant DNA methylation at the earliest stages of oral precancer and finding recurring epigenetic disruption of specific genes/pathways across our analyzed cohort, we see evidence that CpG methylation changes may play a role in oral cancer progression and that global DNA methylation analyses may have significant utility in wider studies that seek to derive biomarkers or potentially druggable targets to improve oral cancer outcomes.
OBJECTIVES: Earlier studies involving a priori gene selection have identified promoter regions deregulated by DNA methylation changes in oral squamous cell cancers (OSCCs) and precancers. Interrogation of global DNA methylation patterns for such specimens has not been reported, though such analyses are needed to uncover novel molecular factors driving disease. MATERIALS AND METHODS: We evaluated global DNA methylation patterns for 30 biopsies obtained from 10 patients undergoing surgical removal of an OSCC or carcinoma in situ (CIS). From a disease field in each patient, we collected (i) dysplastic, (ii) CIS or OSCC, and (iii) adjacent normal biopsies. DNA isolated from each biopsy was profiled for methylation status using the Illumina HumanMethylation27K platform. RESULTS: Our data demonstrate that aberrant methylation of promoter CpG islands exists across oral precancer and OSCC genomes. Non-hierarchical clustering of all methylation data revealed distinct methylation patterns between the normal and the CIS/OSCC tissues (with results for dysplastic biopsies split between groups). Multiple genes exhibiting recurrent aberrant DNA methylation were found for both dysplastic and CIS/OSCC groups, and included enrichment for genes found in the WNT and MAPK signaling pathways. CONCLUSION: In identifying aberrant DNA methylation at the earliest stages of oral precancer and finding recurring epigenetic disruption of specific genes/pathways across our analyzed cohort, we see evidence that CpG methylation changes may play a role in oral cancer progression and that global DNA methylation analyses may have significant utility in wider studies that seek to derive biomarkers or potentially druggable targets to improve oral cancer outcomes.
Authors: Jean-Philippe Foy; Curtis R Pickering; Vassiliki A Papadimitrakopoulou; Jaroslav Jelinek; Steven H Lin; William N William; Mitchell J Frederick; Jing Wang; Wenhua Lang; Lei Feng; Li Zhang; Edward S Kim; You H Fan; Waun K Hong; Adel K El-Naggar; J Jack Lee; Jeffrey N Myers; Jean-Pierre Issa; Scott M Lippman; Li Mao; Pierre Saintigny Journal: Cancer Prev Res (Phila) Date: 2015-09-04
Authors: Rebecca Towle; Ivy F L Tsui; Yuqi Zhu; Sara MacLellan; Catherine F Poh; Cathie Garnis Journal: Cancer Med Date: 2014-07-24 Impact factor: 4.452
Authors: Stephanie M J Fliedner; Uma Shankavaram; Geena Marzouca; Abdel Elkahloun; Ivana Jochmanova; Roland Daerr; W Marston Linehan; Henri Timmers; Arthur S Tischler; Konstantinos Papaspyrou; Jürgen Brieger; Ronald de Krijger; Jan Breza; Graeme Eisenhofer; Zhengping Zhuang; Hendrik Lehnert; Karel Pacak Journal: Neoplasia Date: 2016-09 Impact factor: 5.715