Literature DB >> 24034317

In vitro transcription from plasmid or PCR-amplified DNA.

Julie L Brunelle1, Rachel Green.   

Abstract

This protocol describes the synthesis and purification of RNAs using plasmid DNA or PCR-amplified DNA as a template. This procedure should give NTP-free, full-length RNA for all sizes of RNA. This protocol is derived from Milligan and Uhlenbeck, the classic paper on T7 transcription reactions, with modifications.
© 2013 Elsevier Inc. All rights reserved.

Keywords:  Column purification; In vitro transcription reaction; PCR-amplified DNA; Phenol extract DNA Template; RNA polymerase

Mesh：

Substances：

Year:  2013        PMID： 24034317     DOI： 10.1016/B978-0-12-420037-1.00005-1

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


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