OBJECTIVE: The aim of this study was to investigate effects of mechanical compressive force on differentiation of adipose-derived stem cells (ASCs) in vitro. MATERIALS AND METHODS: Mice ASCs were treated with compressive force (2000 με, 1 Hz) for 2 or 6 h after adipogenic induction for 3 days, then oil red O staining was used to examine oil droplet-filled cells. Adipogenic genes, PPAR-γ1 and APN, were examined by real-time PCR and immunofluorescence (IF) staining was performed to test expression of de-PPAR-γ and ph-PPAR-γ at the protein level. RESULTS: Our data showed that mechanical compressive force reduced numbers of oil droplet-filled cells, and down-regulated mRNA levels of both PPAR-γ1 and APN and protein level of PPAR-γ, in ASCs. CONCLUSIONS: In culture medium containing adipogenic stimuli, mechanical compressive force inhibited adipogenesis of ASCs.
OBJECTIVE: The aim of this study was to investigate effects of mechanical compressive force on differentiation of adipose-derived stem cells (ASCs) in vitro. MATERIALS AND METHODS:Mice ASCs were treated with compressive force (2000 με, 1 Hz) for 2 or 6 h after adipogenic induction for 3 days, then oil red O staining was used to examine oil droplet-filled cells. Adipogenic genes, PPAR-γ1 and APN, were examined by real-time PCR and immunofluorescence (IF) staining was performed to test expression of de-PPAR-γ and ph-PPAR-γ at the protein level. RESULTS: Our data showed that mechanical compressive force reduced numbers of oil droplet-filled cells, and down-regulated mRNA levels of both PPAR-γ1 and APN and protein level of PPAR-γ, in ASCs. CONCLUSIONS: In culture medium containing adipogenic stimuli, mechanical compressive force inhibited adipogenesis of ASCs.
Authors: Yen Kim Luu; Encarnacion Capilla; Clifford J Rosen; Vicente Gilsanz; Jeffrey E Pessin; Stefan Judex; Clinton T Rubin Journal: J Bone Miner Res Date: 2009-01 Impact factor: 6.741