Literature DB >> 24031876

A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel.

Shanlian Qiu1, Jichen Chen, Si Lin, Xinjian Lin.   

Abstract

Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.

Entities:  

Keywords:  Backed gel; DNA; Silver staining

Year:  2012        PMID： 24031876      PMCID： PMC3768845          DOI： 10.1590/S1517-83822012000200029

Source DB:  PubMed          Journal:  Braz J Microbiol        ISSN： 1517-8382            Impact factor:   2.476


  13 in total

Review 1.  DGGE/TGGE a method for identifying genes from natural ecosystems.

Authors:  G Muyzer
Journal:  Curr Opin Microbiol       Date:  1999-06       Impact factor: 7.934

2.  Improved conditions for silver-ammonia staining of DNA in polyacrylamide gel.

Authors:  Wei-Tao Cong; Hong-Zhang He; Zhong-Xin Zhu; Cai-Xue Ye; Xu-Yi Yang; Jung-Kap Choi; Li-Tai Jin; Xiao-Kun Li
Journal:  Electrophoresis       Date:  2010-05       Impact factor: 3.535

3.  Fast and sensitive silver staining of DNA in polyacrylamide gels.

Authors:  B J Bassam; G Caetano-Anollés; P M Gresshoff
Journal:  Anal Biochem       Date:  1991-07       Impact factor: 3.365

4.  An effective method for silver-staining DNA in large numbers of polyacrylamide gels.

Authors:  S O Byun; Q Fang; H Zhou; J G H Hickford
Journal:  Anal Biochem       Date:  2008-10-25       Impact factor: 3.365

5.  16S ribosomal DNA amplification for phylogenetic study.

Authors:  W G Weisburg; S M Barns; D A Pelletier; D J Lane
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

6.  Structure of archaeal communities in membrane-bioreactor and submerged-biofilter wastewater treatment plants.

Authors:  C Gómez-Silván; M Molina-Muñoz; J M Poyatos; A Ramos; E Hontoria; B Rodelas; J González-López
Journal:  Bioresour Technol       Date:  2009-11-30       Impact factor: 9.642

7.  Analysis of microbial communities developed on the fouling layers of a membrane-coupled anaerobic bioreactor applied to wastewater treatment.

Authors:  Kadiya Calderón; Belén Rodelas; Nathalie Cabirol; Jesús González-López; Adalberto Noyola
Journal:  Bioresour Technol       Date:  2011-01-14       Impact factor: 9.642

8.  A simplified silver diammine method for the staining of nucleic acids in polyacrylamide gels.

Authors:  F Vari; K Bell
Journal:  Electrophoresis       Date:  1996-01       Impact factor: 3.535

9.  A silver staining procedure for nucleic acids in polyacrylamide gels without fixation and pretreatment.

Authors:  Z W An; L L Xie; H Cheng; Y Zhou; Q Zhang; X G He; H S Huang
Journal:  Anal Biochem       Date:  2009-05-12       Impact factor: 3.365

10.  Assessing the diversity of bacterial communities associated with plants.

Authors:  Fernando Dini Andreote; João Lúcio Azevedo; Welington Luiz Araújo
Journal:  Braz J Microbiol       Date:  2009-09-01       Impact factor: 2.476
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Authors:  Kaijun Zhao; Jin Xiao; Yu Liu; Shulin Chen; Chunxia Yuan; Aizhong Cao; Frank M You; Donglei Yang; Shengmin An; Haiyan Wang; Xiue Wang
Journal:  Theor Appl Genet       Date:  2018-05-31       Impact factor: 5.699

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Authors:  Ningning Nie; Xiaoyu Ding; Lin Chen; Xun Wu; Yixin An; Chunhui Li; Yanchun Song; Dengfeng Zhang; Zhizhai Liu; Tianyu Wang; Yu Li; Yong-Xiang Li; Yunsu Shi
Journal:  Theor Appl Genet       Date:  2019-09-25       Impact factor: 5.699

3.  Characterization and fine mapping of qkc7.03: a major locus for kernel cracking in maize.

Authors:  Mingtao Yang; Lin Chen; Xun Wu; Xing Gao; Chunhui Li; Yanchun Song; Dengfeng Zhang; Yunsu Shi; Yu Li; Yong-Xiang Li; Tianyu Wang
Journal:  Theor Appl Genet       Date:  2017-11-15       Impact factor: 5.699

4.  Phenotypic characterization of the glossy1 mutant and fine mapping of GLOSSY1 in common wheat (Triticum aestivum L.).

Authors:  Linghong Li; Lingling Chai; Huanwen Xu; Huijie Zhai; Tianya Wang; Mingyi Zhang; Mingshan You; Huiru Peng; Yingyin Yao; Zhaorong Hu; Mingming Xin; Weilong Guo; Qixin Sun; Xiyong Chen; Zhongfu Ni
Journal:  Theor Appl Genet       Date:  2021-01-06       Impact factor: 5.699
  4 in total

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