| Literature DB >> 19013423 |
S O Byun1, Q Fang, H Zhou, J G H Hickford.
Abstract
Silver-staining of nucleic acid has been used for various biological analyses, including polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis. A variety of methods have been described, but these methods are not that effective for staining more than a few PCR-SSCP gels, especially rapidly and with high sensitivity, because they include a number of time-consuming or hazardous manual steps that are often time dependent. Here we report a silver-staining method that can efficiently stain up to 14 gels at one time and with a detection limit of approximately 10 pg of DNA/mm(2), which is comparable to other methods.Entities:
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Year: 2008 PMID： 19013423 DOI： 10.1016/j.ab.2008.10.024
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365