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Literature DB >> 2402510

Protein kinase C is present in human sperm: possible role in flagellar motility.

R Rotem¹, G F Paz, Z T Homonnai, M Kalina, Z Naor.

Abstract

We report the presence of protein kinase C (PKC) in ejaculated human sperm as revealed by enzymatic activity assay and indirect immunohistochemistry. PKC is localized in the equatorial segment and in the principal piece of the tail. Addition of phorbol 12-myristate 13-acetate resulted in increased flagellar motility that was blocked by known PKC inhibitors such as sphingosine, staurosporine, and 1-(5-isoquinoylinylsulfonyl)-2-methylpiperazine. A very good correlation (r = 0.9, P less than 0.001) was found between the percentage of PKC-stained sperm cells and motility. We propose that PKC is involved in the regulation of flagellar motility in human sperm.

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Year: 1990 PMID： 2402510 PMCID： PMC54732 DOI： 10.1073/pnas.87.18.7305

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

29 in total

1. Spermatozoa contain a guanine nucleotide-binding protein ADP-ribosylated by pertussis toxin.

Authors: J K Bentley; D L Garbers; S E Domino; T D Noland; C Van Dop
Journal: Biochem Biophys Res Commun Date: 1986-07-31 Impact factor: 3.575

2. Human semen analysis: seminal plasma and prostatic fluid compositions and their interlations with sperm quality.

Authors: G F Paz; A Sofer; Z T Homonnai; P F Kraicer
Journal: Int J Fertil Date: 1977

3. Evidence for a guanine nucleotide-binding regulatory protein in invertebrate and mammalian sperm. Identification by islet-activating protein-catalyzed ADP-ribosylation and immunochemical methods.

Authors: G S Kopf; M J Woolkalis; G L Gerton
Journal: J Biol Chem Date: 1986-06-05 Impact factor: 5.157

4. Staurosporine, a potent inhibitor of phospholipid/Ca++dependent protein kinase.

Authors: T Tamaoki; H Nomoto; I Takahashi; Y Kato; M Morimoto; F Tomita
Journal: Biochem Biophys Res Commun Date: 1986-03-13 Impact factor: 3.575

5. Metabolism of sea urchin sperm. Interrelationships between intracellular pH, ATPase activity, and mitochondrial respiration.

Authors: R Christen; R W Schackmann; B M Shapiro
Journal: J Biol Chem Date: 1983-05-10 Impact factor: 5.157

6. Phospholipid-sensitive Ca2+-dependent protein kinase system in testis: localization and endogenous substrates.

Authors: K Kimura; N Katoh; K Sakurada; S Kubo
Journal: Endocrinology Date: 1984-12 Impact factor: 4.736

7. Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C.

Authors: H Hidaka; M Inagaki; S Kawamoto; Y Sasaki
Journal: Biochemistry Date: 1984-10-09 Impact factor: 3.162

8. Distribution and activation of protein kinase C in the rat testis tissue.

Authors: H Nikula; Z Naor; M Parvinen; I Huhtaniemi
Journal: Mol Cell Endocrinol Date: 1987-01 Impact factor: 4.102

9. Sphingosine inhibition of protein kinase C activity and of phorbol dibutyrate binding in vitro and in human platelets.

Authors: Y A Hannun; C R Loomis; A H Merrill; R M Bell
Journal: J Biol Chem Date: 1986-09-25 Impact factor: 5.157

10. The voltage-sensitive Na+/H+ exchange in sea urchin spermatozoa flagellar membrane vesicles studied with an entrapped pH probe.

Authors: H C Lee
Journal: J Biol Chem Date: 1985-09-05 Impact factor: 5.157

14 in total

1. Phosphoproteomic and Functional Analyses Reveal Sperm-specific Protein Changes Downstream of Kappa Opioid Receptor in Human Spermatozoa.

Authors: Itziar Urizar-Arenaza; Nerea Osinalde; Vyacheslav Akimov; Michele Puglia; Luz Candenas; Francisco Maria Pinto; Iraia Muñoa-Hoyos; Marta Gianzo; Roberto Matorras; Jon Irazusta; Blagoy Blagoev; Nerea Subiran; Irina Kratchmarova
Journal: Mol Cell Proteomics Date: 2019-01-08 Impact factor: 5.911

Review 2. Ion channels, phosphorylation and mammalian sperm capacitation.

Authors: Pablo E Visconti; Dario Krapf; José Luis de la Vega-Beltrán; Juan José Acevedo; Alberto Darszon
Journal: Asian J Androl Date: 2011-05 Impact factor: 3.285

3. Sperm capacitation in humans is transient and correlates with chemotactic responsiveness to follicular factors.

Authors: A Cohen-Dayag; I Tur-Kaspa; J Dor; S Mashiach; M Eisenbach
Journal: Proc Natl Acad Sci U S A Date: 1995-11-21 Impact factor: 11.205

4. Parallel modulation of receptor for activated C kinase 1 and protein kinase C-alpha and beta isoforms in brains of morphine-treated rats.

Authors: P V Escribá; J A García-Sevilla
Journal: Br J Pharmacol Date: 1999-05 Impact factor: 8.739

5. Participation of the Cl-/HCO(3)- exchangers SLC26A3 and SLC26A6, the Cl- channel CFTR, and the regulatory factor SLC9A3R1 in mouse sperm capacitation.

Authors: Julio C Chávez; Enrique O Hernández-González; Eva Wertheimer; Pablo E Visconti; Alberto Darszon; Claudia L Treviño
Journal: Biol Reprod Date: 2012-01-19 Impact factor: 4.285