Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Use of repetitive DNA probes as physical mapping strategy in Caenorhabditis elegans.

Literature DB >> 2402436

Use of repetitive DNA probes as physical mapping strategy in Caenorhabditis elegans.

G Cangiano¹, H Ameer, R Waterston, A La Volpe.

Abstract

A method for linking genomic sequences cloned in yeast artificial chromosomes (YACs) has been tested using Caenorhabditis elegans as a model system. Yeast clones carrying YACs with repeated sequences were selected from a C. elegans genomic library, total DNA was digested with restriction enzymes, transferred to nylon membranes and probed with a variety of repetitive DNA probes. YAC clones that overlap share common bands with one or more repetitive DNA probes. In 159 YAC clones tested with one restriction enzyme and six probes 28 overlapping clones were detected. The advantages and limitations of this method for construction of YAC physical maps is discussed.

Entities: Species

Mesh：

Substances：
DNA Probes

Year: 1990 PMID： 2402436 PMCID： PMC332126 DOI： 10.1093/nar/18.17.5077

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

10 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresis.

Authors: R Anand; A Villasante; C Tyler-Smith
Journal: Nucleic Acids Res Date: 1989-05-11 Impact factor: 16.971

2 in total

1. Repetitive DNA sequences located in the terminal portion of the Caenorhabditis elegans chromosomes.

Authors: G Cangiano; A La Volpe
Journal: Nucleic Acids Res Date: 1993-03-11 Impact factor: 16.971

2. Opa-like repeats in the genome of the Medfly Ceratitis capitata.

Authors: I Sidén-Kiamos; G Favia; D Artiaco; G Saccone; M Furia; L C Polito; C Louis
Journal: Genetica Date: 1993 Impact factor: 1.082

2 in total

Use of repetitive DNA probes as physical mapping strategy in Caenorhabditis elegans.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresis.

3. Structure, evolution and properties of a novel repetitive DNA family in Caenorhabditis elegans.

4. Software for genome mapping by fingerprinting techniques.

5. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.

6. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

7. pEMBL: a new family of single stranded plasmids.

8. Genome linking with yeast artificial chromosomes.

9. Human Xq24-Xq28: approaches to mapping with yeast artificial chromosomes.

10. A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes.

1. Repetitive DNA sequences located in the terminal portion of the Caenorhabditis elegans chromosomes.

2. Opa-like repeats in the genome of the Medfly Ceratitis capitata.