AIM: Previously, we showed that radioimmunotherapy (RIT) for cryptococcal infections using radioactively labeled antibodies recognizing the cryptococcal capsule reduced fungal burden and prolonged survival of mice infected with Cryptococcus neoformans. Here, we investigate the effects of RIT on bystander mammalian cells. MATERIALS & METHODS: Heat-killed C. neoformans bound to anticapsular antibodies, unlabeled or labeled with the β-emitter rhenium-188 (16.9-h half-life) or the α-emitter bismuth-213 (46-min half-life), was incubated with macrophage-like J774.16 cells or epithelial-like Chinese hamster ovary cells. Lactate dehydrogenase activity, crystal violet uptake, reduction of tetrazolium dye (2,3)-bis-(2-methoxy-4-nitro-5-sulfenyl)-(2H)-terazolium-5-carboxanilide and nitric oxide production were measured. RESULTS: The J774.16 and Chinese hamster ovary cells maintained membrane integrity, viability and metabolic activity following exposure to radiolabeled C. neoformans. CONCLUSION: RIT of C. neoformans is a selective therapy with minimal effects on host cells and these results are consistent with observations that RIT-treated mice with cryptococcal infection lacked RIT-related pathological changes in lungs and brain tissues.
AIM: Previously, we showed that radioimmunotherapy (RIT) for cryptococcal infections using radioactively labeled antibodies recognizing the cryptococcal capsule reduced fungal burden and prolonged survival of mice infected with Cryptococcus neoformans. Here, we investigate the effects of RIT on bystander mammalian cells. MATERIALS & METHODS: Heat-killed C. neoformans bound to anticapsular antibodies, unlabeled or labeled with the β-emitter rhenium-188 (16.9-h half-life) or the α-emitter bismuth-213 (46-min half-life), was incubated with macrophage-like J774.16 cells or epithelial-like Chinese hamster ovary cells. Lactate dehydrogenase activity, crystal violet uptake, reduction of tetrazolium dye (2,3)-bis-(2-methoxy-4-nitro-5-sulfenyl)-(2H)-terazolium-5-carboxanilide and nitric oxide production were measured. RESULTS: The J774.16 and Chinese hamster ovary cells maintained membrane integrity, viability and metabolic activity following exposure to radiolabeled C. neoformans. CONCLUSION: RIT of C. neoformans is a selective therapy with minimal effects on host cells and these results are consistent with observations that RIT-treated mice with cryptococcal infection lacked RIT-related pathological changes in lungs and brain tissues.
Authors: L R Martinez; R A Bryan; C Apostolidis; A Morgenstern; A Casadevall; E Dadachova Journal: Antimicrob Agents Chemother Date: 2006-06 Impact factor: 5.191
Authors: Benjamin J Park; Kathleen A Wannemuehler; Barbara J Marston; Nelesh Govender; Peter G Pappas; Tom M Chiller Journal: AIDS Date: 2009-02-20 Impact factor: 4.177
Authors: Ekaterina Dadachova; Roger W Howell; Ruth A Bryan; Annie Frenkel; Joshua D Nosanchuk; Arturo Casadevall Journal: J Nucl Med Date: 2004-02 Impact factor: 10.057
Authors: Andrew D Schweitzer; Robertha C Howell; Zewei Jiang; Ruth A Bryan; Gary Gerfen; Chin-Cheng Chen; Dennis Mah; Sean Cahill; Arturo Casadevall; Ekaterina Dadachova Journal: PLoS One Date: 2009-09-30 Impact factor: 3.240
Authors: Igor Shuryak; Ruth A Bryan; Jack Broitman; Stephen A Marino; Alfred Morgenstern; Christos Apostolidis; Ekaterina Dadachova Journal: Nucl Med Biol Date: 2015-03-11 Impact factor: 2.408
Authors: Johanna Rivera; Alfred Morgenstern; Frank Bruchertseifer; John F Kearney; Charles L Turnbough; Ekaterina Dadachova; Arturo Casadevall Journal: Antimicrob Agents Chemother Date: 2013-12-30 Impact factor: 5.191
Authors: Qi Hui Sam; Wen Shan Yew; Chaminda J Seneviratne; Matthew Wook Chang; Louis Yi Ann Chai Journal: Front Microbiol Date: 2018-07-25 Impact factor: 5.640
Authors: B van Dijk; K J H Allen; M Helal; H C Vogely; M G E H Lam; J M H de Klerk; H Weinans; B C H van der Wal; E Dadachova Journal: PLoS One Date: 2020-05-14 Impact factor: 3.240