Literature DB >> 24013209

Crystal structure of a substrate-free aspartate transporter.

Sonja Jensen1, Albert Guskov, Stephan Rempel, Inga Hänelt, Dirk Jan Slotboom.   

Abstract

Archaeal glutamate transporter homologs catalyze the coupled uptake of aspartate and three sodium ions. After the delivery of the substrate and sodium ions to the cytoplasm, the empty binding site must reorient to the outward-facing conformation to reset the transporter. Here, we report a crystal structure of the substrate-free transporter GltTk from Thermococcus kodakarensis, which provides insight into the mechanism of this essential step in the translocation cycle.

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Year:  2013        PMID： 24013209     DOI： 10.1038/nsmb.2663

Source DB:  PubMed          Journal:  Nat Struct Mol Biol        ISSN： 1545-9985            Impact factor:   15.369


  20 in total

1.  Evidence for a third sodium-binding site in glutamate transporters suggests an ion/substrate coupling model.

Authors:  H Peter Larsson; Xiaoyu Wang; Bogdan Lev; Isabelle Baconguis; David A Caplan; Nicholas P Vyleta; Hans P Koch; Ana Diez-Sampedro; Sergei Y Noskov
Journal:  Proc Natl Acad Sci U S A       Date:  2010-07-15       Impact factor: 11.205

2.  Na(+):aspartate coupling stoichiometry in the glutamate transporter homologue Glt(Ph).

Authors:  Maarten Groeneveld; Dirk-Jan Slotboom
Journal:  Biochemistry       Date:  2010-05-04       Impact factor: 3.162

3.  Structure of a glutamate transporter homologue from Pyrococcus horikoshii.

Authors:  Dinesh Yernool; Olga Boudker; Yan Jin; Eric Gouaux
Journal:  Nature       Date:  2004-10-14       Impact factor: 49.962

4.  Rigidity of the subunit interfaces of the trimeric glutamate transporter GltT during translocation.

Authors:  Maarten Groeneveld; Dirk-Jan Slotboom
Journal:  J Mol Biol       Date:  2007-06-29       Impact factor: 5.469

5.  Functional characterization of a Na+-dependent aspartate transporter from Pyrococcus horikoshii.

Authors:  Renae M Ryan; Emma L R Compton; Joseph A Mindell
Journal:  J Biol Chem       Date:  2009-04-20       Impact factor: 5.157

6.  Conformational heterogeneity of the aspartate transporter Glt(Ph).

Authors:  Inga Hänelt; Dorith Wunnicke; Enrica Bordignon; Heinz-Jürgen Steinhoff; Dirk Jan Slotboom
Journal:  Nat Struct Mol Biol       Date:  2013-01-20       Impact factor: 15.369

7.  Crystal structure of an asymmetric trimer of a bacterial glutamate transporter homolog.

Authors:  Grégory Verdon; Olga Boudker
Journal:  Nat Struct Mol Biol       Date:  2012-02-12       Impact factor: 15.369

Review 8.  Glutamate uptake.

Authors:  N C Danbolt
Journal:  Prog Neurobiol       Date:  2001-09       Impact factor: 11.685

9.  Cysteine-scanning mutagenesis reveals a highly amphipathic, pore-lining membrane-spanning helix in the glutamate transporter GltT.

Authors:  D J Slotboom; W N Konings; J S Lolkema
Journal:  J Biol Chem       Date:  2001-01-08       Impact factor: 5.157

10.  Flux coupling in a neuronal glutamate transporter.

Authors:  N Zerangue; M P Kavanaugh
Journal:  Nature       Date:  1996-10-17       Impact factor: 49.962
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  49 in total

1.  Substrate-induced rearrangements in glutamate-transporter homologs.

Authors:  Baruch I Kanner
Journal:  Nat Struct Mol Biol       Date:  2013-10       Impact factor: 15.369

2.  Mechanism of transport modulation by an extracellular loop in an archaeal excitatory amino acid transporter (EAAT) homolog.

Authors:  Christopher Mulligan; Joseph A Mindell
Journal:  J Biol Chem       Date:  2013-10-23       Impact factor: 5.157

3.  Investigation of the allosteric coupling mechanism in a glutamate transporter homolog via unnatural amino acid mutagenesis.

Authors:  Erika A Riederer; Francis I Valiyaveetil
Journal:  Proc Natl Acad Sci U S A       Date:  2019-07-22       Impact factor: 11.205

4.  Molecular Determinants of Substrate Specificity in Sodium-coupled Glutamate Transporters.

Authors:  Nechama Silverstein; David Ewers; Lucy R Forrest; Christoph Fahlke; Baruch I Kanner
Journal:  J Biol Chem       Date:  2015-10-16       Impact factor: 5.157

5.  Molecular dynamics simulations elucidate the mechanism of proton transport in the glutamate transporter EAAT3.

Authors:  Germano Heinzelmann; Serdar Kuyucak
Journal:  Biophys J       Date:  2014-06-17       Impact factor: 4.033

6.  Coupling between neurotransmitter translocation and protonation state of a titratable residue during Na ⁺-coupled transport.

Authors:  Ivet Bahar
Journal:  Biophys J       Date:  2014-06-17       Impact factor: 4.033

7.  A novel mutation in SLC1A3 causes episodic ataxia.

Authors:  Kazuhiro Iwama; Aya Iwata; Masaaki Shiina; Satomi Mitsuhashi; Satoko Miyatake; Atsushi Takata; Noriko Miyake; Kazuhiro Ogata; Shuichi Ito; Takeshi Mizuguchi; Naomichi Matsumoto
Journal:  J Hum Genet       Date:  2017-12-05       Impact factor: 3.172

8.  Uptake of amino acids and their metabolic conversion into the compatible solute proline confers osmoprotection to Bacillus subtilis.

Authors:  Adrienne Zaprasis; Monika Bleisteiner; Anne Kerres; Tamara Hoffmann; Erhard Bremer
Journal:  Appl Environ Microbiol       Date:  2014-10-24       Impact factor: 4.792

9.  Low Affinity and Slow Na+ Binding Precedes High Affinity Aspartate Binding in the Secondary-active Transporter GltPh.

Authors:  Inga Hänelt; Sonja Jensen; Dorith Wunnicke; Dirk Jan Slotboom
Journal:  J Biol Chem       Date:  2015-04-28       Impact factor: 5.157

10.  Engineering the glutamate transporter homologue GltPh using protein semisynthesis.

Authors:  Paul J Focke; Alvin W Annen; Francis I Valiyaveetil
Journal:  Biochemistry       Date:  2015-02-17       Impact factor: 3.162
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