BACKGROUND: Genome-wide DNA hypomethylation is known to play important roles in genomic instability and carcinogenesis. Methylation in long interspersed nucleotide element 1 (LINE-1) is a good indicator of the global DNA methylation level within a cell. The aim of this study was to evaluate prognostic significance of LINE-1 hypomethylation in lung adenocarcinoma. METHODS: A consecutive series of 211 lung adenocarcinoma patients who underwent curative resections without any preoperative chemotherapy or radiotherapy at Kumamoto University Hospital between April 2010 and December 2012 were included. The LINE-1 methylation levels were quantified in tumor and noncancerous tissue by Pyrosequencing assay. RESULTS: Higher histologic grade and positive findings for vascular invasion were significantly associated with lower methylation levels. The disease-free survival in the hypomethylation group was significantly shorter than that of the non-hypomethylation group. The prognostic difference was more obvious in advanced cases (stage II, III) than in stage I cases. CONCLUSIONS: The LINE-1 methylation level is associated with histologic grade and vascular invasion of lung adenocarcinoma. Additionally, LINE-1 hypomethylation is a useful biomarker to predict early recurrence of lung adenocarcinoma.
BACKGROUND: Genome-wide DNA hypomethylation is known to play important roles in genomic instability and carcinogenesis. Methylation in long interspersed nucleotide element 1 (LINE-1) is a good indicator of the global DNA methylation level within a cell. The aim of this study was to evaluate prognostic significance of LINE-1 hypomethylation in lung adenocarcinoma. METHODS: A consecutive series of 211 lung adenocarcinomapatients who underwent curative resections without any preoperative chemotherapy or radiotherapy at Kumamoto University Hospital between April 2010 and December 2012 were included. The LINE-1 methylation levels were quantified in tumor and noncancerous tissue by Pyrosequencing assay. RESULTS: Higher histologic grade and positive findings for vascular invasion were significantly associated with lower methylation levels. The disease-free survival in the hypomethylation group was significantly shorter than that of the non-hypomethylation group. The prognostic difference was more obvious in advanced cases (stage II, III) than in stage I cases. CONCLUSIONS: The LINE-1 methylation level is associated with histologic grade and vascular invasion of lung adenocarcinoma. Additionally, LINE-1 hypomethylation is a useful biomarker to predict early recurrence of lung adenocarcinoma.
Authors: Sara Prior; Isabelle R Miousse; Etienne Nzabarushimana; Rupak Pathak; Charles Skinner; Kristy R Kutanzi; Antiño R Allen; Jacob Raber; Alan J Tackett; Martin Hauer-Jensen; Gregory A Nelson; Igor Koturbash Journal: Environ Res Date: 2016-07-14 Impact factor: 6.498
Authors: Young Seok Song; Younghoon Kim; Nam Yun Cho; Han Kwang Yang; Woo Ho Kim; Gyeong Hoon Kang Journal: Gastric Cancer Date: 2015-01-22 Impact factor: 7.370
Authors: So Yeon Park; An Na Seo; Hae Yoen Jung; Jae Moon Gwak; Namhee Jung; Nam-Yun Cho; Gyeong Hoon Kang Journal: PLoS One Date: 2014-06-27 Impact factor: 3.240