Doaa M Abd El-Aziz1. 1. Department of Food Hygiene, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt. doaassiut@yahoo.com
Abstract
OBJECTIVE: To detect Salmonella typhimurium (S. typhimurium), one of the most frequently isolated serovars from food borne outbreaks throughout the world, in retail raw chicken meat and giblets. METHODS: One hundred samples of retail raw chicken meat and giblets (Liver, heart and gizzard) which were collected from Assiut city markets for detection of the organism and by using Duplex PCR amplification of DNA using rfbJ and fliC genes. RESULTS: S. typhimurium was detected at rate of 44%, 40% and 48% in chicken meat, liver and heart, respectively, but not detected in gizzard. CONCLUSIONS: The results showed high incidence of S. typhimurium in the examined samples and greater emphasis should be applied on prevention and control of contamination during processing for reducing food-borne risks to consumers.
OBJECTIVE: To detect Salmonella typhimurium (S. typhimurium), one of the most frequently isolated serovars from food borne outbreaks throughout the world, in retail raw chicken meat and giblets. METHODS: One hundred samples of retail raw chicken meat and giblets (Liver, heart and gizzard) which were collected from Assiut city markets for detection of the organism and by using Duplex PCR amplification of DNA using rfbJ and fliC genes. RESULTS:S. typhimurium was detected at rate of 44%, 40% and 48% in chicken meat, liver and heart, respectively, but not detected in gizzard. CONCLUSIONS: The results showed high incidence of S. typhimurium in the examined samples and greater emphasis should be applied on prevention and control of contamination during processing for reducing food-borne risks to consumers.
Authors: Walid Q Alali; Roman Gaydashov; Elena Petrova; Alexander Panin; Oleg Tugarinov; Alexander Kulikovskii; Dzhemile Mamleeva; Isabel Walls; Michael P Doyle Journal: J Food Prot Date: 2012-08 Impact factor: 2.077