Arthur J Weber1, Christine D Harman. 1. Department of Physiology, Neuroscience Training Program, Michigan State University, East Lansing, Michigan.
Abstract
PURPOSE: We examined the treatment period necessary to restore retinal and visual stability following trauma to the optic nerve. METHODS: Cats received unilateral optic nerve crush and no treatment (NT), treatment of the injured eye with brain-derived neurotrophic factor (BDNF), or treatment of the injured eye combined with treatment of visual cortex for 2 or 4 weeks. After 1-, 2-, 4-, or 6-week survival periods, pattern electroretinograms (PERGs) were obtained and retinal ganglion cell (RGC) survival determined. RESULTS: In the peripheral retina, RGC survival for NT, eye only, and eye + cortex animals was 55%, 78%, and 92%, respectively, at 1 week, and 31%, 60%, and 93%, respectively, at 2 weeks. PERGs showed a similar pattern of improvement. After 4 weeks, RGC survival was 7%, 29%, and 53% in each group, with PERGs in the dual-treated animals similar to the 1- to 2-week animals. For area centralis (AC), the NT, eye only, and eye + cortex animals showed 47%, 78%, and 82% survival, respectively, at 2 weeks, and 13%, 54%, and 81% survival, respectively, at 4 weeks. Removing the pumps at 2 weeks resulted in ganglion cell survival levels of 76% and 74% in the AC at 4 and 6 weeks postcrush, respectively. The PERGs from 2-week treated, but 4- and 6-week survival animals were comparable to those of the 2-week animals. CONCLUSIONS: Treating the entire central visual pathway is important following optic nerve trauma. Long-term preservation of central vision may be achieved with as little as 2 weeks of treatment using this approach.
PURPOSE: We examined the treatment period necessary to restore retinal and visual stability following trauma to the optic nerve. METHODS:Cats received unilateral optic nerve crush and no treatment (NT), treatment of the injured eye with brain-derived neurotrophic factor (BDNF), or treatment of the injured eye combined with treatment of visual cortex for 2 or 4 weeks. After 1-, 2-, 4-, or 6-week survival periods, pattern electroretinograms (PERGs) were obtained and retinal ganglion cell (RGC) survival determined. RESULTS: In the peripheral retina, RGC survival for NT, eye only, and eye + cortex animals was 55%, 78%, and 92%, respectively, at 1 week, and 31%, 60%, and 93%, respectively, at 2 weeks. PERGs showed a similar pattern of improvement. After 4 weeks, RGC survival was 7%, 29%, and 53% in each group, with PERGs in the dual-treated animals similar to the 1- to 2-week animals. For area centralis (AC), the NT, eye only, and eye + cortex animals showed 47%, 78%, and 82% survival, respectively, at 2 weeks, and 13%, 54%, and 81% survival, respectively, at 4 weeks. Removing the pumps at 2 weeks resulted in ganglion cell survival levels of 76% and 74% in the AC at 4 and 6 weeks postcrush, respectively. The PERGs from 2-week treated, but 4- and 6-week survival animals were comparable to those of the 2-week animals. CONCLUSIONS: Treating the entire central visual pathway is important following optic nerve trauma. Long-term preservation of central vision may be achieved with as little as 2 weeks of treatment using this approach.
Authors: Guillermo Parrilla-Reverter; Marta Agudo; Paloma Sobrado-Calvo; Manuel Salinas-Navarro; María P Villegas-Pérez; Manuel Vidal-Sanz Journal: Exp Eye Res Date: 2009-03-04 Impact factor: 3.467
Authors: Thomas V Johnson; Natalie D Bull; David P Hunt; Nephtali Marina; Stanislav I Tomarev; Keith R Martin Journal: Invest Ophthalmol Vis Sci Date: 2009-11-20 Impact factor: 4.799
Authors: Vivek Gupta; Nitin Chitranshi; Veer Gupta; Yuyi You; Rashi Rajput; Joao A Paulo; Mehdi Mirzaei; Maarten van den Buuse; Stuart L Graham Journal: Neuroscience Date: 2022-02-23 Impact factor: 3.708
Authors: Markus H Kuehn; Koren A Lipsett; Marilyn Menotti-Raymond; S Scott Whitmore; Todd E Scheetz; Victor A David; Stephen J O'Brien; Zhongyuan Zhao; Jackie K Jens; Elizabeth M Snella; N Matthew Ellinwood; Gillian J McLellan Journal: PLoS One Date: 2016-05-05 Impact factor: 3.240
Authors: Eline Dekeyster; Emiel Geeraerts; Tom Buyens; Chris Van den Haute; Veerle Baekelandt; Lies De Groef; Manuel Salinas-Navarro; Lieve Moons Journal: PLoS One Date: 2015-11-11 Impact factor: 3.240