Zong-Li Hu1, Ni Li1, Xin Wei1, Li Tang1, Ting-Hua Wang2, Xiao-Ming Chen1. 1. Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China. 2. Institute of Neurological Disease, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
Abstract
AIM: To assess the neuro-protective effect of bone marrow mesenchymal stem cells (BMSCs) on retinal ganglion cells (RGCs) following optic nerve crush in mice. METHODS: C56BL/6J mice were treated with intravitreal injection of PBS, BMSCs, BDNF-interference BMSCs (BIM), and GDNF-interference BMSCs (GIM) following optic nerve crush, respectively. The number of surviving RGCs was determined by whole-mount retinas and frozen sections, while certain mRNA or protein was detected by q-PCR or ELISA, respectively. RESULTS: The density (cell number/mm2) of RGCs was 410.77±56.70 in the retina 21d after optic nerve crush without any treatment, compared to 1351.39±195.97 in the normal control (P<0.05). RGCs in BMSCs treated eyes was 625.07±89.64/mm2, significantly higher than that of no or PBS treatment (P<0.05). While RGCs was even less in the retina with intravitreal injection of BIM (354.07+39.77) and GIM (326.67+33.37) than that without treatment (P<0.05). BMSCs injection improved the internal BDNF expression in retinas. CONCLUSION: Optic nerve crush caused rust loss of RGCs and intravitreally transplanted BMSCs at some extent protected RGCs from death. The effect of BMSCs and level of BDNF in retinas are both related to BDNF and GDNF expression in BMSCs.
AIM: To assess the neuro-protective effect of bone marrow mesenchymal stem cells (BMSCs) on retinal ganglion cells (RGCs) following optic nerve crush in mice. METHODS: C56BL/6J mice were treated with intravitreal injection of PBS, BMSCs, BDNF-interference BMSCs (BIM), and GDNF-interference BMSCs (GIM) following optic nerve crush, respectively. The number of surviving RGCs was determined by whole-mount retinas and frozen sections, while certain mRNA or protein was detected by q-PCR or ELISA, respectively. RESULTS: The density (cell number/mm2) of RGCs was 410.77±56.70 in the retina 21d after optic nerve crush without any treatment, compared to 1351.39±195.97 in the normal control (P<0.05). RGCs in BMSCs treated eyes was 625.07±89.64/mm2, significantly higher than that of no or PBS treatment (P<0.05). While RGCs was even less in the retina with intravitreal injection of BIM (354.07+39.77) and GIM (326.67+33.37) than that without treatment (P<0.05). BMSCs injection improved the internal BDNF expression in retinas. CONCLUSION: Optic nerve crush caused rust loss of RGCs and intravitreally transplanted BMSCs at some extent protected RGCs from death. The effect of BMSCs and level of BDNF in retinas are both related to BDNF and GDNF expression in BMSCs.
Authors: Guillermo Parrilla-Reverter; Marta Agudo; Paloma Sobrado-Calvo; Manuel Salinas-Navarro; María P Villegas-Pérez; Manuel Vidal-Sanz Journal: Exp Eye Res Date: 2009-03-04 Impact factor: 3.467
Authors: Michael Blair; Mary Ellen Pease; John Hammond; Danielle Valenta; Jennifer Kielczewski; Hana Levkovitch-Verbin; Harry Quigley Journal: Invest Ophthalmol Vis Sci Date: 2005-03 Impact factor: 4.799
Authors: Eric Falke; Jonathan Nissanov; Thomas W Mitchell; David A Bennett; John Q Trojanowski; Steven E Arnold Journal: Am J Pathol Date: 2003-10 Impact factor: 4.307
Authors: Alexander A Shpak; Alla B Guekht; Tatiana A Druzhkova; Anna A Troshina; Natalia V Gulyaeva Journal: Mol Vis Date: 2022-05-15 Impact factor: 2.711